Isolation and characterization of poly- and oligosaccharides from the red microalga Porphyridium sp

2)- or (1-->4)-linked d-xylopyranosyl, (1-->3)-linked L-galactopyranosyl, (1-->3)-linked D-glucopyranosyl, and (1-->3)-linked D-glucopyranosyluronic acid and comprises a possible acidic building unit: [(2 or 4)-beta-D-Xylp-(L-->3)]m-alpha-D-Glcp-(1-->3)-alpha-D-GLCPA-(1-->3)-L-Galp(l-->. Attached to the backbone are sulfate groups and nonreducing terminal D-xylopyranosyl and galactopyranosyl residues, which occur at the O-6 positions of Glc-derived moieties in the main chain. -->
ISSN: 0008-6215
DOI: 10.1016/j.carres.2008.11.012
Přístupová URL adresa: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a75503219524da1e5172cd02c3cb29f6
https://doi.org/10.1016/j.carres.2008.11.012
Rights: CLOSED
Přírůstkové číslo: edsair.doi.dedup.....a75503219524da1e5172cd02c3cb29f6
Autor: Robert Glaser, Shimona Geresh, Wang Zhang, Oshrat Levy-Ontman, Shoshana (Malis) Arad, Yoram Tekoah
Rok vydání: 2009
Předmět:
Zdroj: Carbohydrate Research. 344:343-349
ISSN: 0008-6215
DOI: 10.1016/j.carres.2008.11.012
Popis: The current study forms part of an ongoing research effort focusing on the elucidation of the chemical structure of the sulfated extracellular polysaccharide of the red microalga Porphyridium sp. (UTEX 637). We report here on the chemical structure of a fraction separated from an acidic crude extract of the polysaccharide, as investigated by methylation analysis, carboxyl reduction-methylation analysis, desulfation-methylation analysis, partial acid hydrolysis, Smith degradation, together with 1D and 2D (1)H and (13)C NMR spectroscopy. This fraction with a molar mass of 2.39x10(5)g mol(-1) comprised D- and L-Gal, D-Glc, D-Xyl, D-GlcA, and sulfate groups in a molar ratio of 1.0:1.1:2.1:0.2:0.7. The almost linear backbone of the fraction is composed of (1-->2)- or (1-->4)-linked d-xylopyranosyl, (1-->3)-linked L-galactopyranosyl, (1-->3)-linked D-glucopyranosyl, and (1-->3)-linked D-glucopyranosyluronic acid and comprises a possible acidic building unit: [(2 or 4)-beta-D-Xylp-(L-->3)]m-alpha-D-Glcp-(1-->3)-alpha-D-GLCPA-(1-->3)-L-Galp(l-->. Attached to the backbone are sulfate groups and nonreducing terminal D-xylopyranosyl and galactopyranosyl residues, which occur at the O-6 positions of Glc-derived moieties in the main chain.
Databáze: OpenAIRE
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