Genetic micro-heterogeneity of Leishmania major in emerging foci of zoonotic cutaneous leishmaniasis in Tunisia

Autor: Carola Schweynoch, Hanène Attia, Gabriele Schönian, Rabiaa M. Sghaier, Ghada Mkannez, Sadok Chlif, Tesfaye Gelanew, Dhafer Laouini, Koussay Dellagi, Fatma Z. Guerfali, Aymen Bali, Nabil Belhaj-Hamida
Přispěvatelé: Université de Tunis El Manar (UTM), Laboratoire de Transmission, Contrôle et Immunobiologie des Infections - Laboratory of Transmission, Control and Immunobiology of Infection (LR11IPT02), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Laboratoire d'Epidémiologie Médicale, Institut Pasteur de Tunis, This work was supported by an NIH/NIAID/DMID Grant Number 5P50AI074178 and the Tunisian Ministry of Higher Education and Research (LR11 IPT02) for KD and DL., We are grateful to Prof. A. Ben Salah and Mr. A. Zaatour for kindly providing us with L. major isolates from the field. We thank Ms. R. Yazidi and Ms. S. Chaâbane for their valuable technical help. We are thankful to Dr. K. Ben-Aissa for her English and grammar corrections and to Dr. J Bettaieb for her advices on statistical analyses.
Rok vydání: 2016
Předmět:
0301 basic medicine
MESH: Leishmaniasis
Cutaneous/transmission
MESH: Leishmania major/genetics
Endemic Diseases
MESH: Tunisia/epidemiology
MESH: Genome
Protozoan
Zoonoses
Leishmania major
MESH: Animals
Phylogeny
MESH: Phylogeny
Genetics
Phylogenetic tree
MESH: Genetic Heterogeneity
3. Good health
Emerging focus
MESH: Endemic Diseases
MESH: Leishmania major/classification
Infectious Diseases
MESH: Multilocus Sequence Typing
Parasitic disease
Genetic structure
Microsatellite
MESH: Zoonoses
Microbiology (medical)
Tunisia
MESH: Psychodidae/parasitology
MESH: Leishmaniasis
Cutaneous/epidemiology
Leishmaniasis
Cutaneous
Locus (genetics)
Biology
MESH: DNA
Protozoan/genetics
Microbiology
MESH: Genetic Loci
03 medical and health sciences
Genetic Heterogeneity
medicine
Animals
Humans
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Typing
Allele
Molecular Biology
MESH: Life Cycle Stages/genetics
Ecology
Evolution
Behavior and Systematics
Alleles
Multi-locus microsatellite typing
MESH: Leishmaniasis
Cutaneous/parasitology
Life Cycle Stages
MESH: Humans
MESH: Alleles
DNA
Protozoan
biology.organism_classification
medicine.disease
MESH: Leishmania major/isolation & purification
030104 developmental biology
Genetic Loci
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Old focus
MESH: Microsatellite Repeats
Heterogeneity
Psychodidae
MESH: Leishmania major/growth & development
Genome
Protozoan
Zoonotic cutaneous leishmaniasis
Microsatellite Repeats
Multilocus Sequence Typing
Zdroj: Infection, Genetics and Evolution
Infection, Genetics and Evolution, Elsevier, 2016, 43, pp.179-185. ⟨10.1016/j.meegid.2016.04.032⟩
ISSN: 1567-7257
1567-1348
DOI: 10.1016/j.meegid.2016.04.032⟩
Popis: International audience; Tunisia is endemic for zoonotic cutaneous leishmaniasis (ZCL), a parasitic disease caused by Leishmania (L.) major. ZCL displays a wide clinical polymorphism, with severe forms present more frequently in emerging foci where naive populations are dominant. In this study, we applied the multi-locus microsatellite typing (MLMT) using ten highly informative and discriminative markers to investigate the genetic structure of 35 Tunisian Leishmania (L.) major isolates collected from patients living in five different foci of Central Tunisia (two old and three emerging foci). Phylogenetic reconstructions based on genetic distances showed that nine of the ten tested loci were homogeneous in all isolates with homozygous alleles, whereas one locus (71AT) had a 58/64-bp bi-allelic profile with an allele linked to emerging foci. Promastigote-stage parasites with the 58-bp allele tend to be more resistant to in vitro complement lysis. These results, which stress the geographical dependence of the genetic micro-heterogeneity, may improve our understanding of the ZCL epidemiology and clinical outcome.
Databáze: OpenAIRE
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