A Rapid Field-Deployable Reverse Transcription-Insulated Isothermal Polymerase Chain Reaction Assay for Sensitive and Specific Detection of Bluetongue Virus
| Autor: | Aruna Ambagala, D. J. Johnson, P.-Y. A. Lee, Eileen N. Ostlund, Oliver Lung, S. Pahari, Mathew Fisher, John Pasick |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Serotype Orbivirus General Veterinary General Immunology and Microbiology Reverse Transcriptase Polymerase Chain Reaction RNA Reoviridae General Medicine Ruminants Biology biology.organism_classification Culicoides Virology Sensitivity and Specificity Virus Reverse transcriptase law.invention 03 medical and health sciences 030104 developmental biology law Animals RNA Viral Polymerase chain reaction Bluetongue virus |
| Zdroj: | Transboundary and emerging diseases. 64(2) |
| ISSN: | 1865-1682 |
| Popis: | Summary Bluetongue is a non-contagious, haemorrhagic, Culicoides-borne disease of ruminants. The causative agent, bluetongue virus (BTV), is a member of the Orbivirus genus of the Reoviridae family. So far, 26 BTV serotypes have been identified worldwide. The global distribution of bluetongue has been expanding, and rapid detection of BTV, preferably in the field, is critical for timely implementation of animal movement restrictions and vector control measures. To date, many laboratory-based, molecular assays for detection of BTV have been developed. These methods require the samples to be shipped to a central laboratory with sophisticated instruments and highly skilled technicians to perform the assays, conduct analyses and interpret the results. Here, we report the development and evaluation of a rapid, portable, user-friendly, pan-BTV reverse transcription-insulated isothermal polymerase chain reaction (RT-iiPCR) assay that can potentially be used in low-resource field conditions. The total length of the assay was |
| Databáze: | OpenAIRE |
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