Imbalance towards Th1 pathway predominance in purpura nephritis with proteinuria
| Autor: | Eishin Oki, Etsuro Ito, Tadaatsu Imaizumi, Hidemi Yoshida, Hiroshi Tanaka, Kazushi Tsuruga, Tomomi Aizawa-Yashiro, Shojiro Watanabe |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Adolescent IgA Vasculitis Helper T lymphocyte Urinary system GATA3 Transcription Factor Real-Time Polymerase Chain Reaction chemistry.chemical_compound Internal medicine Biopsy medicine Humans RNA Messenger Child Creatinine Nephritis Proteinuria medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction business.industry hemic and immune systems Glomerulonephritis Th1 Cells medicine.disease Endocrinology chemistry Nephrology Child Preschool Pediatrics Perinatology and Child Health Female Renal biopsy medicine.symptom T-Box Domain Proteins business |
| Zdroj: | Pediatric Nephrology. 26:2253-2258 |
| ISSN: | 1432-198X 0931-041X |
| Popis: | Although recent reports suggest a possible role for an imbalance in Th1 and Th2 proinflammatory cytokines in the development and progression of glomerulonephritis, there is little information on Th1 or Th2 predominance in Henoch-Schönlein purpura nephritis (HSPN). Since T-bet and GATA-3 are transcriptional factors that regulate the differentiation of helper T lymphocytes into Th1 and Th2, we examined the relative mRNA expression of T-bet and GATA-3 in the urinary sediment of children with proteinuric HSPN by real-time quantitative PCR. Eight consecutive patients with proteinuric HSPN (4 with grade IIIa and 4 with grade IIIb according to the International Study of Kidney Disease in Children criteria) and 20 healthy subjects were enrolled in this study. The relative expression level of T-bet was significantly higher in the urinary sediment of patients with HSPN at presentation than in that of the healthy controls (p = 0.021), while the relative expression of GATA-3 was significantly lower in the urinary sediment of patients than in that of controls (p = 0.002). Urinary mRNA expression of T-bet correlated with the urinary protein/creatinine ratio (r = 0.608, p = 0.013), and correlated inversely with serum level of total protein (r = -0.574, p = 0.020). Moreover, a significantly increased intensity of T-bet immunostaining was observed in the glomeruli in the biopsy specimen of all study patients. All patients received immunosuppressive therapy. Repeat measurements of urinary mRNA expression of T-bet and GATA-3 after treatment revealed that the expression of T-bet in patients had significantly decreased relative to the baseline (p = 0.003), while the expression of GATA-3 remained static. In a patient subjected to a post-treatment renal biopsy, the increased intensity of immunostaining of T-bet had clearly diminished following immunosuppressive treatment, in accordance with a significant decrease in urinary mRNA expression of T-bet. These observations suggest that patients with proteinuric HSPN demonstrate increased T-bet and depressed GATA-3 expression in the urinary sediment, indicating a possible shift in Th1/Th2 balance towards Th1 predominance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|