Riboflavin synthesis from gaseous nitrogen and carbon dioxide by a hybrid inorganic-biological system
| Autor: | Rebecca S. Sherbo, Pamela A. Silver, Daniel G. Nocera |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Proceedings of the National Academy of Sciences. 119 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Microbes can provide a more sustainable and energy-efficient method of food and nutrient production compared to plant and animal sources, but energy-intensive carbon (e.g., sugars) and nitrogen (e.g., ammonia) inputs are required. Gas-fixing microorganisms that can grow on H 2 from renewable water splitting and gaseous CO 2 and N 2 offer a renewable path to overcoming these limitations but confront challenges owing to the scarcity of genetic engineering in such organisms. Here, we demonstrate that the hydrogen-oxidizing carbon- and nitrogen-fixing microorganism Xanthobacter autotrophicus grown on a CO 2 /N 2 /H 2 gas mixture can overproduce the vitamin riboflavin (vitamin B 2 ). We identify plasmids and promoters for use in this bacterium and employ a constitutive promoter to overexpress riboflavin pathway enzymes. Riboflavin production is quantified at 15 times that of the wild-type organism. We demonstrate that riboflavin overproduction is maintained when the bacterium is grown under hybrid inorganic-biological conditions, in which H 2 from water splitting, along with CO 2 and N 2 , is fed to the bacterium, establishing the viability of the approach to sustainably produce food and nutrients. |
| Databáze: | OpenAIRE |
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