TANGO1 assembles into rings around COPII coats at ER exit sites

Autor: Seetharaman Parashuraman, Timo Zimmermann, Ishier Raote, María Ortega Bellido, Chong Zhang, Marianella Pirozzi, David B. Melville, Vivek Malhotra
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Recercat. Dipósit de la Recerca de Catalunya
instname
The Journal of cell biology 216 (2017): 901–909. doi:10.1083/jcb.201608080
info:cnr-pdr/source/autori:Raote, Ishier; Ortega Bellido, Maria; Pirozzi, Marinella; Zhang, Chong; Melville, David; Parashuraman, Seetharaman; Zimmermann, Timo; Malhotra, Vivek/titolo:TANGO1 assembles into rings around COP II coats at ER exit sites/doi:10.1083%2Fjcb.201608080/rivista:The Journal of cell biology/anno:2017/pagina_da:901/pagina_a:909/intervallo_pagine:901–909/volume:216
The Journal of Cell Biology
DOI: 10.1083/jcb.201608080
Popis: TANGO1 interacts with COPII components to generate a transport carrier for export of large cargo from the endoplasmic reticulum. Raote et al. show that TANGO1 molecules assemble to form a closed ribbon structure that encircles COPII components.
TANGO1 (transport and Golgi organization 1) interacts with CTAGE5 and COPII components Sec23/Sec24 and recruits ERGIC-53 (endoplasmic reticulum [ER]–Golgi intermediate compartment 53)–containing membranes to generate a mega-transport carrier for export of collagens and apolipoproteins from the ER. We now show that TANGO1, at the ER, assembles in a ring that encircles COPII components. The C-terminal, proline-rich domains of TANGO1 molecules in the ring are initially tilted onto COPII coats but appear to be pushed apart as the carrier grows. These findings lend support to our suggestion that growth of transport carriers for exporting bulky cargoes requires addition of membranes and not simply COPII-mediated accretion of a larger surface of ER. TANGO1 remains at the neck of the newly forming transport carrier, which grows in size by addition of ERGIC-53–containing membranes to generate a transport intermediate for the export of bulky collagens.
Databáze: OpenAIRE