Enzyme-functionalized biomimetic apatites: concept and perspectives in view of innovative medical approaches

Autor: Iris Trick, Nicolas Vandecandelaere, Christina G. Weber, Anke Burger-Kentischer, Tanja Maucher, Christophe Drouet, Michaela Mueller
Přispěvatelé: Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut Fraunhofer-Gesellschaft (GERMANY), Institut National Polytechnique de Toulouse - INPT (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Universität Stuttgart (GERMANY), Centre Interuniversitaire de Recherche et d'Ingénierie des Matériaux - CIRIMAT (Toulouse, France), Fraunhofer Institute for Interfacial Engineering and Biotechnology (Fraunhofer IGB), Fraunhofer (Fraunhofer-Gesellschaft), Stuttgart University, Centre interuniversitaire de recherche et d'ingenierie des matériaux (CIRIMAT), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC), Publica, Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Calcium Phosphates
Cytotoxicity
Sample processing
Molecular Conformation
02 engineering and technology
01 natural sciences
Bone tissue engineering
Apatite
[SPI.MAT]Engineering Sciences [physics]/Materials
Biomimetic Materials
Osteogenesis
Apatites
Enzyme Stability
Materials Testing
chemistry.chemical_classification
Surface reactivity
hydroxyapatite
[CHIM.MATE]Chemical Sciences/Material chemistry
021001 nanoscience & nanotechnology
visual_art
Bone surgery
Drug delivery
visual_art.visual_art_medium
[SDV.IB]Life Sciences [q-bio]/Bioengineering
0210 nano-technology
Hybrid material
Cell Survival
Surface Properties
Matériaux
Biomedical Engineering
Biophysics
Bioengineering
Nanotechnology
010402 general chemistry
Bacterial Physiological Phenomena
Cell Line
Biomaterials
Humans
Biomimetic apatite
Particle Size
Subtilisin
Hybrid
0104 chemical sciences
Nanostructures
Ingénierie biomédicale
Enzyme Activation
antibacterial
Enzyme
chemistry
Bone Substitutes
Muramidase
Zdroj: Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine, Springer Verlag, 2014, Vol. 25 (n° 3), pp. 595-606. ⟨10.1007/s10856-013-5097-9⟩
ISSN: 0957-4530
1573-4838
DOI: 10.1007/s10856-013-5097-9⟩
Popis: International audience; Biomimetic nanocrystalline calcium-deficient apatite compounds are particularly attractive for the setup of bioactive bone-repair scaffolds due to their high similarity to bone mineral in terms of chemical composition, structural and substructural features. As such, along with the increasingly appealing development of moderate temperature engineered routes for sample processing, they have widened the armamentarium of orthopedic and maxillofacial surgeons in the field of bone tissue engineering. This was made possible by exploiting the exceptional surface reactivity of biomimetic apatite nanocrystals, capable of easily exchanging ions or adsorbing (bio)molecules, thus leading to highly-versatile drug delivery systems. In this contribution we focus on the preparation of hybrid materials combining biomimetic nanocrystallineapatites and enzymes (lysozyme and subtilisin). This paper reports physico-chemical data as well as cytotoxicity evaluations towards Cal-72 osteoblast-like cells and finally antimicrobial assessments towards selected strains of interest in bone surgery. Biomimetic apatite/enzyme hybrids could be prepared in varying buffers. They were found to be non-cytotoxic toward osteoblastic cells and the enzymes retained their biological activity (e.g. bond cleavage or antibacterial properties) despite the immobilization and drying processes. Release properties were also examined. Beyond these illustrative examples, the concept of biomimetic apatites functionalized with enzymes is thus shown to be useable in practice, e.g. for antimicrobial purposes, thus widening possible therapeutic perspectives.
Databáze: OpenAIRE
Externí odkaz: