Glucocorticoid receptor expression in human bronchial epithelial cells: effects of smoking and COPD

Autor:	P. T. W. Van Hal, Henk C. Hoogsteden, A. O. Brinkmann, C A Berrevoets, Marjan A. Versnel, P. W. C. Adriaansen-Soeting, M. M. Verheggen
Přispěvatelé:	Pulmonary Medicine, Immunology, Developmental Biology
Rok vydání:	1998
Předmět:	medicine.medical_specialty education Immunology Bronchi Inflammation Dexamethasone Cell Line Receptors Glucocorticoid Smoking Glucocorticoid receptor Internal medicine lcsh:Pathology medicine Humans Lung Diseases Obstructive Binding site Glucocorticoid receptors Receptor Aged COPD Binding Sites business.industry Chronic obstructive pulmonary disease Epithelial Cells Cell Biology Middle Aged medicine.disease respiratory tract diseases Dissociation constant Endocrinology Cell culture medicine.symptom business Human bronchial epithelial cells Research Article lcsh:RB1-214 medicine.drug
Zdroj:	Mediators of Inflammation, Vol 7, Iss 4, Pp 275-281 (1998) Mediators of Inflammation Mediators of Inflammation, 7, 275-281. Hindawi Publishing Corporation
ISSN:	1466-1861 0962-9351
DOI:	10.1080/09629359890965
Popis:	Previously, we found that inflammatory mediators modulated the number and binding affinity of glucocorticoid receptors (GR) in human bronchial epithelial cell lines . In this study we investigated whether smoking and chronic obstructive pulmonary disease (COPD), both characterized by airway inflammation with increased levels of inflammatory mediators , affect GR characteristics in cultured human bronchial epithelial cells (HBEC). A statisticallys ignificant difference was found be tween the dissociation constant (Kd) values in HBEC from smoking (Kd= 0.98 ± 0.08 nM;n=6) and nonsmoking controls (Kd= 0.76 ± 0.10 nM,p=0.03;n=5), but no significant difference was found between the mean number of binding sites . Our results are the first indication that cultured HBEC from smokers possess GR with a lower binding affinity. This may result from the in flammation found in the airways from smokers . Further more, these results provide furthere vidence that the bronchial epithelium may be an actual target for inhaled glucocor ticoid therapy.
Databáze:	OpenAIRE
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