Synthetic beta-endorphin-like peptide immunorphin binds to non-opioid receptors for beta-endorphin on T lymphocytes
| Autor: | T. N. Lepikhova, Natalia Malkova, Valery M. Lipkin, Vladimir P. Zav'yalov, Elena V. Navolotskaya, T. A. Zargarova |
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| Rok vydání: | 2002 |
| Předmět: |
Physiology
Immunoglobulin gamma-Chains T-Lymphocytes Peptide (+)-Naloxone Biochemistry Human Immunoglobulin G Cellular and Molecular Neuroscience Endocrinology medicine Humans Amino Acid Sequence Receptor chemistry.chemical_classification Heavy chain Chemistry beta-Endorphin Molecular biology Peptide Fragments Opioid Immunorphin Receptors Opioid Immunoglobulin Constant Regions Oligopeptides medicine.drug |
| Zdroj: | Peptides. 22(12) |
| ISSN: | 0196-9781 |
| Popis: | The synthetic decapeptide H-SLTCLVKGFY-OH (termed immunorphin) corresponding to the sequence 364-373 of the CH3 domain of human immunoglobulin G heavy chain was found to compete with [125I]beta-endorphin for high-affinity receptors on T lymphocytes from the blood of healthy donors (K(i) = 0.6 nM). Besides immunorphin, its synthetic fragments H-Val-Lys-Gly-Phe-Tyr-OH (K(i) = 15 nM), H-Leu-Val-Lys-Gly-Phe-Tyr-OH (K(i) = 8.0 nM), H-Cys-Leu-Val-Lys-Gly-Phe-Tyr-OH (K(i) = 3.4 nM), H-Thr-Cys-Leu-Val-Lys-Gly-Phe-Tyr-OH (K(i) = 2.2 nM), H-Leu-Thr-Cys-Leu-Val-Lys-Gly-Phe-Tyr-OH (K(i) = 1.0 nM) possessed the ability to inhibit specific binding of [125I]beta-endorphin to T lymphocytes. Tests of the specificity of the receptors revealed that they are not sensitive to naloxone and Met-enkephalin, i.e. they are not opioid receptors. K(d) values characterizing the specific binding of 125I- labeled immunorphin and its fragment H-Val-Lys-Gly-Phe-Tyr-OH to the receptors have been determined to be 7.4 nM and 36.3 nM, respectively. |
| Databáze: | OpenAIRE |
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