PUFA-dependent alteration of oxidative parameters of a canine mastocytoma cell line
| Autor: | Sandra Schmutzler, Herbert Fuhrmann, Julia Schumann, L. Bachmann |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Linoleic acid
Wasp Venoms Canine Mastocytoma Biology Lipid peroxidation chemistry.chemical_compound Dogs Cell Line Tumor medicine Animals Mast Cells chemistry.chemical_classification General Veterinary Mastocytoma medicine.disease Mast cell Eicosapentaenoic acid medicine.anatomical_structure Biochemistry chemistry Fatty Acids Unsaturated Intercellular Signaling Peptides and Proteins Arachidonic acid Lipid Peroxidation Peptides Reactive Oxygen Species Oxidation-Reduction DNA Damage Polyunsaturated fatty acid |
| Zdroj: | Acta Veterinaria Hungarica. 58:453-464 |
| ISSN: | 1588-2705 0236-6290 |
| DOI: | 10.1556/avet.58.2010.4.6 |
| Popis: | Mast cells play a key role in the immune response. Thereby, the balance of oxidative metabolism is of importance in mast cell mediator synthesis and release. Fatty acids may modify mast cell function in several ways. In this study, we investigated the influence of polyunsaturated fatty acids (PUFAs) on oxidative parameters of a canine mastocytoma cell line. C2 cells were cultured in media supplemented with linoleic acid, arachidonic acid, alpha-linolenic acid and eicosapentaenoic acid, respectively. Production of reactive oxygen species (ROS) as well as lipid peroxides was tested. Furthermore, stressor-induced DNA damage was measured. Exposure of the cells to PUFAs resulted in a significant increase in the synthesis of both ROS and lipid peroxides. Distinct differences between the PUFAs tested underline the impact of the unsaturation degree of fatty acids as well as the position of double bonds on mast cells. |
| Databáze: | OpenAIRE |
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