Studies of Cicatricial Pemphigoid Autoantibodies Using Direct Immunoelectron Microscopy and Immunoblot Analysis
| Autor: | Louis Dubertret, Jean-Claude Roujeau, Jean Revuz, Christophe Bedane, Patrick Combemale, Liliane Intrator, Jean-Marie Bonnetblanc, Philippe Bernard, Véronique Lecerf, Catherine Prost |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Adult
Male Epidermolysis bullosa acquisita Pathology medicine.medical_specialty Pemphigoid Immunoelectron microscopy Blotting Western Fluorescent Antibody Technique Dermatology Biochemistry Cicatrix Pemphigoid Bullous medicine Humans Cicatricial pemphigoid Molecular Biology Aged Autoantibodies Skin Dermoepidermal junction Aged 80 and over Skin Diseases Vesiculobullous Chemistry Mouth Mucosa Cell Biology Middle Aged medicine.disease Lamina lucida Microscopy Electron Female Lamina densa Bullous pemphigoid |
| Zdroj: | Journal of Investigative Dermatology. 94(5):630-635 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/1523-1747.ep12876206 |
| Popis: | We studied 11 consecutive patients with classical cicatricial pemphigoid (CP) using direct immunoelectron microscopy (IEM) and Western immunoblotting analysis. Direct IEM performed in the skin or gingival mucosa revealed in all 11 CP patients that immunoglobulins and complement deposits were usually thick and discontinuous along the dermoepidermal junction, mostly localized on the lamina densa and occasionally in the lamina lucida. By direct IEM, the ultra-structural aspect in CP differs from the pattern observed in bullous pemphigoid (BP) and from that of chronic epidermolysis bullosa acquisita (EBA). Nine CP patients were studied by Western immunoblotting and, of these nine, only two had detectable anti-basement membrane zone (BMZ) antibodies by indirect immunofluorescence on salt-split skin. By immunoblotting performed on protein extracts of heat-separated epidermis, eight out of the nine CP sera specifically reacted with two protein bands of approximately 230 – 240 kD and 180 kD, similar to those recognized by BP sera in co-migration experiments. By immunoblotting on skin BMZ extracts, none of these nine CP sera recognized the 290-kD major polypeptide of EBA antigen. Taken together, these results suggest that, in CP, the target-antigen, as identified on immunoblots, is similar to BP antigen, but with an abnormal expression within the dermoepidermal junction of patients, which may in part explain the scarring course of the disease. |
| Databáze: | OpenAIRE |
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