RNA splicing: group I intron crystal structures reveal the basis of splice site selection and metal ion catalysis

Autor:	Scott A. Strobel, Mary R. Stahley
Rok vydání:	2006
Předmět:	Ions Models Molecular Genetics Spliceosome Binding Sites Splice site mutation RNA Splicing Group I intron splicing Intron Group II intron Biology Catalysis Introns Polypyrimidine tract Metals Structural Biology RNA splicing Nucleic Acid Conformation RNA Group I catalytic intron Molecular Biology
Zdroj:	Current Opinion in Structural Biology. 16:319-326
ISSN:	0959-440X
DOI:	10.1016/j.sbi.2006.04.005
Popis:	The group I intron has served as a model for RNA catalysis since its discovery 25 years ago. Four recently determined high-resolution crystal structures complement extensive biochemical studies on this system. Structures of the Azoarcus, Tetrahymena and bacteriophage Twort group I introns mimic different states of the splicing or ribozyme reaction pathway and provide information on splice site selection and metal ion catalysis. The 5'-splice site is selected by formation of a conserved G.U wobble pair between the 5'-exon terminus and the intron. The 3'-splice site is identified through stacking of three base triples, in which the middle triple contains the conserved terminal nucleotide of the intron, OmegaG. The structures support a two-metal-ion mechanism for group I intron splicing that might have corollaries to group II intron and pre-mRNA splicing by the spliceosome.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a74e2f420ff203ebacfed58eb3a5873d https://doi.org/10.1016/j.sbi.2006.04.005 Zobrazit plný text záznamu Full Text from ScienceDirect