Artery targeted photothrombosis widens the vascular penumbra, instigates peri-infarct neovascularization and models forelimb impairments
Autor: | Theresa A. Jones, Michael R. Williamson, Andrew K. Dunn, Daniella M. Palmberg, S. M. Shams Kazmi, Taylor A. Clark, Colin T. Sullender, Brittany L. Speetles |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Brain Infarction Male medicine.medical_specialty Light Neovascularization Physiologic lcsh:Medicine Article Neovascularization 03 medical and health sciences Mice 0302 clinical medicine Internal medicine Cortex (anatomy) Forelimb medicine Animals lcsh:Science Stroke Multidisciplinary business.industry Penumbra lcsh:R Motor Cortex Thrombosis Arteries medicine.disease Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cerebral blood flow Cerebrovascular Circulation Cardiology Female lcsh:Q medicine.symptom business 030217 neurology & neurosurgery Artery Motor cortex |
Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | The photothrombotic stroke model generates localized and reproducible ischemic infarcts that are useful for studying recovery mechanisms, but its failure to produce a substantial ischemic penumbra weakens its resemblance to human stroke. We examined whether a modification of this approach, confining photodamage to arteries on the cortical surface (artery-targeted photothrombosis), could better reproduce aspects of the penumbra. Following artery-targeted or traditional photothrombosis to the motor cortex of mice, post-ischemic cerebral blood flow was measured using multi-exposure speckle imaging at 6, 48, and 120 h post-occlusion. Artery-targeted photothrombosis produced a more graded penumbra at 48 and 120 h. The density of isolectin B4+ vessels in peri-infarct cortex was similarly increased after both types of infarcts compared to sham at 2 weeks. These results indicate that both models instigated post-ischemic vascular structural changes. Finally, we determined whether the strength of the traditional photothrombotic approach for modeling upper-extremity motor impairments extends to the artery-targeted approach. In adult mice that were proficient in a skilled reaching task, small motor-cortical infarcts impaired skilled-reaching performance for up to 10 days. These results support that artery-targeted photothrombosis widens the penumbra while maintaining the ability to create localized infarcts useful for modeling post-stroke impairments. |
Databáze: | OpenAIRE |
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