Blood biochemistry and hematological changes in rats after administration of a mixture of three anesthetic agents
| Autor: | Naoto Toyota, Mio Hiramatsu, Kazuto Yamashita, Toshihiko Watanabe, Yuichiro Ochiai, Hidetomo Iwano, Atushi Baba, Hiroshi Yokota |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Blood Glucose Male Butorphanol medetomidine Hematocrit Pharmacology anesthesia 03 medical and health sciences Eating Hemoglobins Laboratory Animal Science White blood cell medicine Animals atipamezole General Veterinary medicine.diagnostic_test Full Paper business.industry Body Weight Atipamezole Medetomidine Anesthetics Combined Blood Cell Count Rats 030104 developmental biology medicine.anatomical_structure midazolam Anesthetic Midazolam butorphanol Hemoglobin business medicine.drug |
| Zdroj: | The Journal of Veterinary Medical Science |
| Popis: | Currently, given the concerns regarding animal welfare, it is required that anesthesia or analgesia be used during surgery in experimental animals. Therefore, it is important to understand how anesthesia affects the health conditions of experimental animals. In this study, rat blood biochemistry and hematological changes were examined following administration of a mixture of three anesthetic agents-medetomidine, midazolam and butorphanol (MMB). One of three MMB dose combinations was subcutaneously administered to rats. After 1 hr, rats were treated with atipamezole, to reverse the anesthetic effects. Blood biochemistry and hematological parameters were assessed at 1, 4 and 24 hr post-MMB treatment. We also recorded body weight and food intake at 0, 2, 4, 6 and 24 hr post-MMB administration. Following MMB administration, transient increases were observed in glucose (GLUC) levels, hematocrit (HCT) values and hemoglobin (HGB) levels, whereas transient decreases were observed in total protein (TP) content and white blood cell (WBC) counts. Most of these parameters returned to control values 24 hr following MMB administration. Additionally, body weight and food intake decreased in MMB-treated rats. In conclusion, intermediate and high doses of MMB changed some blood biochemistry and hematological parameters, body weight and food intake. In contrast, low-dose MMB did not cause these effects. Therefore, depending on the experimental design, MMB may influence the results of studies that use laboratory animals. Consequently, anesthetic agents used in laboratory animals should be chosen based on detailed knowledge of their pharmacological effects. |
| Databáze: | OpenAIRE |
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