Determination of Aloesin and Aloeresin A for the Detection of Aloe in Beverages
| Autor: | Patrizia Ferraboschi, Enrica Bosisio, Mario Dell'Agli, F. Giavarini, Giovanni Galli |
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| Rok vydání: | 2007 |
| Předmět: |
Chromatography
biology Chemistry Silica gel Alcoholic Beverages Aloe ferox Extraction (chemistry) Food Contamination General Chemistry biology.organism_classification High-performance liquid chromatography Beverages Plant Leaves chemistry.chemical_compound Column chromatography Glucosides Chromones Sephadex Aloe General Agricultural and Biological Sciences Hplc method Chromatography High Pressure Liquid Control methods |
| Zdroj: | Journal of Agricultural and Food Chemistry. 55:3363-3367 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf070182h |
| Popis: | This work describes a sensitive high-performance liquid chromatography (HPLC) method for the quantification of aloesin and aloeresin A in alcoholic beverages containing aloe as a flavoring agent. The compounds were prepared from Aloe ferox juice. Sephadex LH20 and ion-exchange resin AG1X2 column chromatography were used for aloesin. Aloeresin A was obtained by Sephadex LH20 and silica gel column chromatography followed by purification on Discovery DSC-18 solid-phase extraction tubes. A 98 mg amount of aloesin (>99% purity) and 34 mg of aloeresin A (>98% purity) were recovered from 2.5 g of aloe juice. The HPLC method was validated, and intra- and interday performances were established. In-house validation was carried out by analyzing samples of beverages with and without aloe as a flavoring agent. |
| Databáze: | OpenAIRE |
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