Determination of Cannabinoids in Breast Milk Using QuEChERS and Ultra-Performance Liquid Chromatography and Tandem Mass Spectrometry
| Autor: | Rhea S Ramnarine, Carl E. Wolf, Justin L. Poklis |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Health
Toxicology and Mutagenesis Cannabinol Breast milk Toxicology Tandem mass spectrometry Quechers 01 natural sciences Analytical Chemistry Matrix (chemical analysis) 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Limit of Detection Tandem Mass Spectrometry 030225 pediatrics mental disorders medicine Environmental Chemistry Cannabidiol Humans Cannabis Detection limit Chemical Health and Safety Chromatography Milk Human Cannabinoids 010401 analytical chemistry Extraction (chemistry) 0104 chemical sciences Substance Abuse Detection chemistry medicine.drug |
| Zdroj: | Journal of analytical toxicology. 43(9) |
| ISSN: | 1945-2403 |
| Popis: | Use of marijuana and cannabinoids has been on the rise in recent years, including in childbearing women. This has resulted in cannabinoids being more frequently identified in breast milk as a result of its high lipid content and cannabinoids having a high lipophilicity, thereby exposing the breastfeeding infant to cannabinoids and other marijuana constituents. Presented is a method for the analysis of Δ9-tetrahydrocannabinol (THC), cannabinol (CBN) and cannabidiol (CBD) in breast milk. THC, CBN, CBD and their isotopically labeled standards were extracted from breast milk using a modified QuEChERS method and analyzed using ultra-performance liquid chromatography and tandem mass spectrometry. As a result of the high lipid content of breast milk, saponification of the lipids was necessary to improve overall extraction efficiency. The process efficiency percentage for THC, CBD and CBN are 55%, 80% and 25%, respectively. The recovery percentage for THC, CBD and CBN are 95%, 118% and 85%, respectively. The matrix effect percentage for THC, CBD and CBN are 53%, 66% and 26%, respectively. Linearity was assessed from 1 to 100 ng/mL for THC, CBN and CBD and had r2 > 0.996. Validation controls were prepared at 1, 3, 20, 80 and 300 ng/mL (dilution control), and the bias was determined to be less than ±20% with %CVs |
| Databáze: | OpenAIRE |
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