β-Glucanase specific expression in the parotid gland of transgenic mice
| Autor: | Yong-Liang Zhang, Jun-yun Zhou, Li-zheng Guan, Yu-ping Sun, Qingyan Jiang, Jing-lan Wang, Qianyun Xi, Gang Shu |
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| Rok vydání: | 2012 |
| Předmět: |
Genetically modified mouse
Saliva Glycoside Hydrolases Transgene Mice Transgenic Biology Mice stomatognathic system Genetics medicine Animals Parotid Gland Northern blot Salivary Proteins and Peptides Microinjection Glucans Southern blot Glucanase Molecular biology Parotid gland stomatognathic diseases medicine.anatomical_structure Gene Expression Regulation Animal Science and Zoology Agronomy and Crop Science Paenibacillus Biotechnology |
| Zdroj: | Transgenic research. 22(4) |
| ISSN: | 1573-9368 |
| Popis: | The feasibility of using the pig parotid secretory protein promoter to drive the β-glucanase transgene expression in mouse parotid glands was examined in this study. The parotid gland-specific vector expressing β-glucanase gene (GLU, from Paenibacillus polymyxa CP7) was constructed. Transgenic mice were produced by the pronuclear microinjection. Both PCR and Southern blot analysis showed that the mice carried the β-glucanase gene and the β-glucanase gene could be stably inherited. Furthermore, RT-PCR and northern blot analysis indicated that it was specifically expressed in the parotid. The β-glucanase activity in the saliva was found to be 0.18 U/mL. After feeding a diet containing 2 % β-glucan, the average daily gain of transgenic was significantly higher than non-transgenic mice. The crude protein and crude fat concentration in faeces of transgenic mice were significantly reduced compared with that of the non-transgenic mice. These results suggest that the successful expression of foreign β-glucanase in the animal parotid would offer a promising biological approach to reduce the anti-nutritional effect of β-glucans in feed. |
| Databáze: | OpenAIRE |
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