CD73 Promotes Age-Dependent Accretion of Atherosclerosis
| Autor: | Nadia R. Sutton, David J. Pinsky, Matthew C. Hyman, Imran M. Rashid, Daniel R. Goldstein, Diane Bouis, Alexandra L. McCubbrey, Annie Mei, Kris M. Mann |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Adenosine monophosphate medicine.medical_specialty Aorta Thoracic Age dependent Article Accretion (finance) Mice chemistry.chemical_compound Internal medicine medicine Animals Ectonucleotidase 5'-Nucleotidase Chromatography High Pressure Liquid Mice Knockout Chemistry Gene Expression Regulation Developmental Atherosclerosis Flow Cytometry Immunohistochemistry Adenosine Mice Inbred C57BL Disease Models Animal Phenotype Endocrinology RNA Female Cardiology and Cardiovascular Medicine medicine.drug |
| Zdroj: | Arterioscler Thromb Vasc Biol |
| ISSN: | 1524-4636 1079-5642 |
| Popis: | Objective: CD73 is an ectonucleotidase which catalyzes the conversion of AMP (adenosine monophosphate) to adenosine. Adenosine has been shown to be anti-inflammatory and vasorelaxant. The impact of ectonucleotidases on age-dependent atherosclerosis remains unclear. Our aim was to investigate the role of CD73 in age-dependent accumulation of atherosclerosis. Approach and results: Mice doubly deficient in CD73 and ApoE (apolipoprotein E; ( cd73 − /− /apoE −/− ) were generated, and the extent of aortic atherosclerotic plaque was compared with apoE −/− controls at 12, 20, 32, and 52 weeks. By 12 weeks of age, cd73 −/− /apoE −/− mice exhibited a significant increase in plaque (1.4±0.5% of the total vessel surface versus 0.4±0.1% in apoE −/− controls, P P P cd73 −/− /apoE −/− mice and apoE −/− controls. A reduction in lipolysis in CD73 competent mice was observed, even with similar plasma lipid levels ( cd73 −/− /apoE −/− versus apoE −/− at 12 weeks [16.2±0.7 versus 9.5±1.4 nmol glycerol/well], 32 weeks [24.1±1.5 versus 7.4±0.4 nmol/well], and 52 weeks [13.8±0.62 versus 12.7±2.0 nmol/well], P Conclusions: At early time points, CD73 exerts a subtle antiatherosclerotic influence, but with age, the pattern reverses, and the presence of CD73 promoted suppression of lipid catabolism. |
| Databáze: | OpenAIRE |
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