A Changing Gastric Environment Leads to Adaptation of Lipopolysaccharide Variants in Helicobacter pylori Populations during Colonization
| Autor: | Helene Kling Bäckhed, Britta Björkholm, Staffan Normark, Anna Skoglund, Christina Nilsson, Lars Engstrand |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Lipopolysaccharides
Infectious Diseases/Gastrointestinal Infections Atrophic gastritis lcsh:Medicine Epitope Infectious Diseases/Bacterial Infections Epitopes Mice lcsh:Science Stomach cancer Pathogen 0303 health sciences education.field_of_study Multidisciplinary Stomach Hydrogen-Ion Concentration Middle Aged 3. Good health Phenotype Infectious Diseases Gastroenterology and Hepatology/Gastrointestinal Infections medicine.anatomical_structure Disease Progression Microbiology/Cellular Microbiology and Pathogenesis Research Article Population Biology Microbiology 03 medical and health sciences Lewis Blood Group Antigens Antigen medicine Animals Humans education Aged DNA Primers 030304 developmental biology Inflammation Helicobacter pylori 030306 microbiology lcsh:R Microbiology/Medical Microbiology biology.organism_classification medicine.disease digestive system diseases Case-Control Studies Immunology lcsh:Q |
| Zdroj: | PLoS ONE PLoS ONE, Vol 4, Iss 6, p e5885 (2009) |
| ISSN: | 1932-6203 |
| Popis: | The human gastric pathogen Helicobacter pylori colonizes the stomachs of half of the human population, and causes development of peptic ulcer disease and gastric adenocarcinoma. H. pylori-associated chronic atrophic gastritis (ChAG) with loss of the acid-producing parietal cells, is correlated with an increased risk for development of gastric adenocarcinoma. The majority of H. pylori isolates produce lipopolysaccharides (LPS) decorated with human-related Lewis epitopes, which have been shown to phase-vary in response to different environmental conditions. We have characterized the adaptations of H. pylori LPS and Lewis antigen expression to varying gastric conditions; in H. pylori isolates from mice with low or high gastric pH, respectively; in 482 clinical isolates from healthy individuals and from individuals with ChAG obtained at two time points with a four-year interval between endoscopies; and finally in isolates grown at different pH in vitro. Here we show that the gastric environment can contribute to a switch in Lewis phenotype in the two experimental mouse models. The clinical isolates from different human individuals showed that intra-individual isolates varied in Lewis antigen expression although the LPS diversity was relatively stable within each individual over time. Moreover, the isolates demonstrated considerable diversity in the levels of glycosylation and in the sizes of fucosylated O-antigen chains both within and between individuals. Thus our data suggest that different LPS variants exist in the colonizing H. pylori population, which can adapt to changes in the gastric environment and provide a means to regulate the inflammatory response of the host during disease progression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|