Interleukin-1, tumor necrosis factor-alpha, and transforming growth factor-beta 1 and integrative meniscal repair: influences on meniscal cell proliferation and migration
| Autor: | Nicole E. Rothfusz, Rebecca E. Wilusz, Farshid Guilak, J B Weinberg, Amy L. McNulty, Katherine M Riera |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Survival Swine Immunology Meniscus (anatomy) Menisci Tibial Tissue Culture Techniques Transforming Growth Factor beta1 03 medical and health sciences 0302 clinical medicine Rheumatology Cell Movement Immunology and Allergy Medicine Animals Osteoarthritic knee Cells Cultured 030304 developmental biology Cell Proliferation 030203 arthritis & rheumatology 0303 health sciences Wound Healing Microscopy Confocal biology Cell growth business.industry Tumor Necrosis Factor-alpha Interleukin Transforming growth factor beta Meniscal repair Biomechanical Phenomena medicine.anatomical_structure biology.protein Cancer research Tumor necrosis factor alpha Female business Wound healing Research Article Interleukin-1 |
| Zdroj: | Arthritis Research & Therapy |
| ISSN: | 1478-6362 1478-6354 |
| Popis: | Introduction Interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) are up-regulated in injured and osteoarthritic knee joints. IL-1 and TNF-α inhibit integrative meniscal repair; however, the mechanisms by which this inhibition occurs are not fully understood. Transforming growth factor-β1 (TGF-β1) increases meniscal cell proliferation and accumulation, and enhances integrative meniscal repair. An improved understanding of the mechanisms modulating meniscal cell proliferation and migration will help to improve approaches for enhancing intrinsic or tissue-engineered repair of the meniscus. The goal of this study was to examine the hypothesis that IL-1 and TNF-α suppress, while TGF-β1 enhances, cellular proliferation and migration in cell and tissue models of meniscal repair. Methods A micro-wound assay was used to assess meniscal cell migration and proliferation in response to the following treatments for 0, 24, or 48 hours: 0 to 10 ng/mL IL-1, TNF-α, or TGF-β1, in the presence or absence of 10% serum. Proliferated and total cells were fluorescently labeled and imaged using confocal laser scanning microscopy and the number of proliferated, migrated, and total cells was determined in the micro-wound and edges of each image. Meniscal cell proliferation was also assessed throughout meniscal repair model explants treated with 0 or 10 ng/mL IL-1, TNF-α, or TGF-β1 for 14 days. At the end of the culture period, biomechanical testing and histological analyses were also performed. Statistical differences were assessed using an ANOVA and Newman-Keuls post hoc test. Results IL-1 and TNF-α decreased cell proliferation in both cell and tissue models of meniscal repair. In the presence of serum, TGF-β1 increased outer zone cell proliferation in the micro-wound and in the cross section of meniscal repair model explants. Both IL-1 and TNF-α decreased the integrative shear strength of repair and extracellular matrix deposition in the meniscal repair model system, while TGF-β1 had no effect on either measure. Conclusions Meniscal cell proliferation in vivo may be diminished following joint injury due to the up-regulation of inflammatory cytokines, thereby limiting native cellular repair of meniscal lesions. Therefore, therapies that can promote meniscal cell proliferation have promise to enhance meniscal repair and improve tissue engineering strategies. |
| Databáze: | OpenAIRE |
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