Several nuclear genes control both male sterility and mitochondrial protein synthesis inNicotiana sylvestris protoclones
| Autor: | Fernand Vedel, Veronique Vitart, D. Prat, R. De Paepe, P. Chetrit, F. Ambard-Bretteville |
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| Rok vydání: | 1990 |
| Předmět: |
Mitochondrial DNA
Nuclear gene Sterility Biology DNA Mitochondrial Culture Techniques Tobacco Gene expression Genetics Cloning Molecular Molecular Biology Gene Crosses Genetic Plant Proteins Protoplasts Reproduction Genetic Complementation Test fungi Cytoplasmic male sterility Genetic Variation food and beverages Protoplast biology.organism_classification Mitochondria Plants Toxic Electrophoresis Polyacrylamide Gel Nicotiana sylvestris |
| Zdroj: | Molecular and General Genetics MGG. 222:206-210 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/bf00633819 |
| Popis: | Male sterile plants appeared in the progeny of three fertile plants obtained after one cycle of protoplast culture from a fertile botanical line and two androgenetic lines of Nicotiana sylvestris. These plants showed the same foliar and floral abnormalities as the cytoplasmic male sterile (cms) mitochondrial variants obtained after two cycles of culture. We show that male sterility in these plants is controlled by three independent nuclear genes, ms1, ms2 and ms3, while no changes can be seen in the mitochondrial genome. However, differences were found between the in organello mitochondrial protein synthesis patterns of male sterile and parent plants. Two reproducible changes were observed: the presence of a new 20 kDa polypeptide and the absence of a 40 kDa one. Such variations were described previously in mitochondrial protein synthesis patterns of the cms lines. Fertile hybrids of male sterile plants showed normal synthesis patterns. The male sterile plants are thus mutated in nuclear genes involved in changes observed in mitochondrial protein synthesis patterns. |
| Databáze: | OpenAIRE |
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