Segmentation of nearly isotropic overlapped tracks in photomicrographs using successive erosions as watershed markers
| Autor: | Sandro Guedes, Lothar Ratschbacher, Wagner Massayuki Nakasuga, Alexandre Fioravante de Siqueira |
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| Rok vydání: | 2019 |
| Předmět: |
Histology
Watershed Computer science Diallyl phthalate business.industry Isotropy Pattern recognition 030206 dentistry 02 engineering and technology 021001 nanoscience & nanotechnology 03 medical and health sciences Medical Laboratory Technology 0302 clinical medicine Digital image processing Segmentation Artificial intelligence Anatomy 0210 nano-technology business Instrumentation Test data |
| Zdroj: | Microscopy Research and Technique. 82:1706-1719 |
| ISSN: | 1097-0029 1059-910X |
| DOI: | 10.1002/jemt.23336 |
| Popis: | INTRODUCTION Procedures for measuring and counting tracks are time-consuming and involve practical problems. The precision of automatic counting methods is not satisfactory yet; the major challenges are distinguishing tracks and material defects, identifying small tracks and defects of similar size, and detecting overlapping tracks. MATERIALS AND METHODS Here, we address the overlapping tracks issue using the algorithm Watershed Using Successive Erosions as Markers (WUSEM), which combines the watershed transform, morphological erosions and labeling to separate regions in photomicrographs. We tested this method in two data sets of diallyl phthalate (DAP) photomicrographs and compared the results when counting manually and using the classic watershed and H-watershed transforms. RESULTS The mean automatic/manual efficiency counting ratio when using WUSEM in the test data sets is 0.97 ± 0.11. CONCLUSION WUSEM shows reliable results when used in photomicrographs presenting almost isotropic objects. Also, diameter and eccentricity criteria may be used to increase the reliability of this method. |
| Databáze: | OpenAIRE |
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