Galleria mellonella as a model system to study virulence potential of mucormycetes and evaluation of antifungal treatment
| Autor: | Verena Naschberger, Cornelia Lass-Flörl, Caroline Hörtnagl, Margarita Semis, Ulrike Binder, Denise Grässle, Elisabeth Maurer, Patrizia Moser, Esther Segal, Michaela Lackner |
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| Rok vydání: | 2018 |
| Předmět: |
Mucorales
Posaconazole animal structures Antifungal Agents Pyridines Virulence nystatin-intralipid Microbial Sensitivity Tests mucormycosis Microbiology 03 medical and health sciences Drug Resistance Fungal Amphotericin B Nitriles medicine antifungal susceptibility Animals 030304 developmental biology Mucor 0303 health sciences biology 030306 microbiology Mucormycosis fungi in vivo model General Medicine Triazoles biology.organism_classification medicine.disease Galleria mellonella Rhizomucor Lepidoptera Disease Models Animal Infectious Diseases Nystatin Larva Original Article Rhizopus medicine.drug |
| Zdroj: | Medical Mycology |
| ISSN: | 1460-2709 |
| Popis: | Mucorales can cause cutaneous to deep-seated infections, mainly in the immunocompromised host, resulting in high mortality rates due to late and inefficient treatment. In this study, Galleria mellonella larvae were evaluated as a heterologous invertebrate host to study pathogenicity of clinically relevant mucormycetes (Rhizopus spp., Rhizomucor spp., Lichtheimia spp., Mucor spp.). All tested species were able to infect G. mellonella larvae. Virulence potential was species-specific and correlated to clinical relevance. Survival of infected larvae was dependent on (a) the species (growth speed and spore size), (b) the infection dose, (c) the incubation temperature, (d) oxidative stress tolerance, and (e) iron availability in the growth medium. Moreover, we exploited the G. mellonella system to determine antifungal efficacy of liposomal amphotericin B, posaconazole, isavuconazole, and nystatin-intralipid. Outcome of in vivo treatment was strongly dependent upon the drug applied and the species tested. Nystatin-intralipid exhibited best activity against Mucorales, followed by posaconazole, while limited efficacy was seen for liposomal amphotericin B and isavuconazole. Pharmacokinetic properties of the tested antifungals within this alternative host system partly explain the limited treatment efficacy. In conclusion, G. mellonella represents a useful invertebrate infection model for studying virulence of mucormycetes, while evaluation of treatment response was limited. |
| Databáze: | OpenAIRE |
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