The C-terminal variable region specifies the dynamic properties of Arabidopsis microtubule-associated protein MAP65 isotypes
| Autor: | Jindriska Fiserova, Zdenek Opatrny, Patrick J. Hussey, Andrei Smertenko, Despina Kaloriti, Hsin-Yu Chang |
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| Rok vydání: | 2008 |
| Předmět: |
Microtubule-associated protein
Immunoblotting Arabidopsis Plant Science Microtubules Microtubule polymerization Microtubule Arabidopsis thaliana Protein Isoforms Central spindle Mitosis Research Articles Plant Proteins Genetics Microscopy Confocal biology Arabidopsis Proteins food and beverages Cell Biology biology.organism_classification Recombinant Proteins Cell biology Microtubule-Associated Proteins Cytokinesis Protein Binding |
| Zdroj: | The Plant cell. 20(12) |
| ISSN: | 1040-4651 |
| Popis: | The microtubule-associated protein, MAP65, is a member of a family of divergent microtubule-associated proteins from different organisms generally involved in maintaining the integrity of the central spindle in mitosis. The dicotyledon Arabidopsis thaliana and the monocotyledon rice (Oryza sativa) genomes contain 9 and 11 MAP65 genes, respectively. In this work, we show that the majority of these proteins fall into five phylogenetic clades, with the greatest variation between clades being in the C-terminal random coil domain. At least one Arabidopsis and one rice isotype is within each clade, indicating a functional specification for the C terminus. In At MAP65-1, the C-terminal domain is a microtubule binding region (MTB2) harboring the phosphorylation sites that control its activity. The At MAP65 isotypes show differential localization to microtubule arrays and promote microtubule polymerization with variable efficiency in a MTB2-dependent manner. In vivo studies demonstrate that the dynamics of the association and dissociation of different MAP65 isotypes with microtubules can vary up to 10-fold and that this correlates with their ability to promote microtubule polymerization. Our data demonstrate that the C-terminal variable region, MTB2, determines the dynamic properties of individual isotypes and suggest that slower turnover is conditional for more efficient microtubule polymerization. |
| Databáze: | OpenAIRE |
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