Simvastatin Improves the Jaw Bone Microstructural Defect Induced by High Cholesterol Diet in Rats by Regulating Autophagic Flux
| Autor: | Shengyun Huang, Zhenggang Chen, Yanjun Cui, Xuxia Wang, Zhou Jianhua, Dongsheng Zhang, Ma Li, Hengkun Wang, Quanjiang Dong, Lili Wang, Xiaoli Gao, Qiu Jianzhong |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty China Simvastatin Article Subject lcsh:Medicine Mandible General Biochemistry Genetics and Molecular Biology Proinflammatory cytokine Cholesterol Dietary Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Autophagy Animals Hypolipidemic Agents General Immunology and Microbiology biology Chemistry Cholesterol lcsh:R Jaw bone General Medicine Diet Rats 030104 developmental biology Endocrinology RANKL Beijing biology.protein Immunohistochemistry Flux (metabolism) Research Article medicine.drug |
| Zdroj: | BioMed Research International, Vol 2018 (2018) BioMed Research International |
| ISSN: | 2314-6141 |
| Popis: | Objective. The objective of this study is to evaluate the effect of simvastatin on the jaw bone microstructural defect and autophagy in rats with high cholesterol diet (HCD). Methods. Male Sprague-Dawley rats were fed a standard rodent chow (NC group) or a high cholesterol diet for 32 weeks and the HCD-fed rats were treated with vehicle (HC group) or simvastatin (5 mg/kg orally daily for 8 weeks, HC + SIM group, and n=10/group). The static histomorphometric changes in the jaw bone tissues in individual rats were evaluated. The relative levels of OPG, RANKL, NF-κB, LC3, and p62 in the jaw bone tissues were determined by quantitative RT-PCR and/or immunohistochemistry. Results. Compared with the NC group, the HC groups had lower trabecular bone volume, trabecular thickness and trabecular number, and increased ratios of RANKL/OPG in the jaw bone, accompanied by enhanced NF-κB activation and autophagy. Simvastatin treatment inhabited these changes, including the decreased levels of serum proinflammatory cytokines and increased autophagy. Conclusion. Simvastatin treatment could inhibit the hyperlipidemia-induced jaw bone microstructural defect in rats by increasing autophagic flux. |
| Databáze: | OpenAIRE |
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