Semisynthesis of a Bacterium with Non-canonical Cell-Wall Cross-Links
| Autor: | Bill Webb, Jason S. Chen, David A. Dik, Peter G. Schultz, Nan Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Phenylalanine
Peptide Peptidoglycan 010402 general chemistry medicine.disease_cause 01 natural sciences Biochemistry Catalysis Cell wall chemistry.chemical_compound Colloid and Surface Chemistry Cell Wall Escherichia coli medicine Sulfones Cell Engineering chemistry.chemical_classification biology General Chemistry biology.organism_classification Semisynthesis 0104 chemical sciences chemistry Non canonical Biophysics Bacterial outer membrane Bacteria |
| Zdroj: | Journal of the American Chemical Society. 142:10910-10913 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/jacs.0c02956 |
| Popis: | The cell wall is an elaborate framework of peptidoglycan that serves to protect the bacterium against osmotic challenge. This exoskeleton is composed of repeating saccharides covalently cross-linked by peptide stems. The general structure of the cell wall is widely conserved across diverse Gram-negative bacteria. To begin to explore the biological consequence of introducing non-canonical cross-links into the cell wall of Escherichia coli, we generated a bacterium where up to 31% of the cell-wall cross-links are formed by a non-enzymatic reaction between a sulfonyl fluoride and an amino group. Bacteria with these non-canonical cell-wall cross-links achieve a high optical density in culture, divide and elongate successfully, and display no loss of outer membrane integrity. This work represents a first step in the design of bacteria with non-canonical "synthetic" cell walls. |
| Databáze: | OpenAIRE |
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