Next-generation sequencing-based QTL mapping for unravelling causative genes associated with melon fruit quality traits
| Autor: | Galil Tzuri, C. Jiao, Zhangjun Fei, Einat Bar, Efraim Lewinsohn, Amit Gur, Nadia Lombardi, Y. Burger, R. Harel-Beja, Adi Doron-Faigenboim, Guy Kol, L. Mao, M. Kenigswald, Yaakov Tadmor, U. Sa’ar, Shery Lev, Vitaly Portnoy, A. A. Schaffer, N. Katzir, Itay Gonda, Navot Galpaz, Elazar Fallik, Y. Xu, James J. Giovannoni, Omer Barad |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics Melon business.industry food and beverages Horticulture Biology Quantitative trait locus 01 natural sciences DNA sequencing Biotechnology 03 medical and health sciences 030104 developmental biology Family-based QTL mapping Cucumis melo Genotyping by sequencing RNA-seq business Gene 010606 plant biology & botany |
| Zdroj: | Acta horticulturae 1151 (2017): 9–16. doi:10.17660/ActaHortic.2017.1151.3 info:cnr-pdr/source/autori:Portnoy V., Gonda I., Galpaz N., Tzuri G., Lev S., Kenigswald M., Fei Z., Barad O., Harel-Beja R., Doron-Faigenboim A., Bar E., Sáar U., Xu Y., Lombardi N., Mao L., Jiao C., Kol G., Gur A., Fallik E., Tadmor Y., Burger Y., Schaffer A.A., Giovannoni J., Lewinsohn E., Katzir N./titolo:Next-generation sequencing-based QTL mapping for unravelling causative genes associated with melon fruit quality traits/doi:10.17660%2FActaHortic.2017.1151.3/rivista:Acta horticulturae/anno:2017/pagina_da:9/pagina_a:16/intervallo_pagine:9–16/volume:1151 |
| ISSN: | 2406-6168 0567-7572 |
| Popis: | High resolution QTL mapping of genetic factors affecting fruit quality traits in melon (Cucumis melo) was achieved in two projects. Next-generation sequencing (NGS), coupled with unique genetic materials, was used to attain high resolution mapping, in both studies. The first project was based on the RNA-seq of mature fruits of a Recombinant Inbred Lines (RIL) population derived from a cross between PI 414723 and 'Dulce'. The second project was based on genotyping-by-sequencing (GBS) analyses of F3 plants derived from a cross between 'Noy Amid' and 'Dulce'. In each project, QTL analysis was conducted using approximately 60,000 SNP markers that were detected by either GBS or RNA-seq. In the first project, phenotyping and metabolic profiling included fruit quality traits associated with taste, aroma and color while the second project focused on flesh and rind color, ethylene and the formation of the fruit abscission zone. Evidence of the high resolution of the analysis was provided in cases where QTLs for selected fruit traits were mapped to an interval of 1- 20 genes that included the known underlying causative genes. These cases included genes associated with the accumulation of specific aroma volatiles and fruit fleshcolor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|