Synergistic Effect of Combined Treatment with Longan Flower Extract and 5-Fluorouracil on Colorectal Cancer Cells
| Autor: | Hsin-Ping Chang, Yuan-Chiang Chung, Chih-Hsien Chen, Han-Lung Chang, Chih-Ping Hsu, Szu-Jung Chen, Chih-Cheng Lin, Jui-Ling Chou |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Antimetabolites Antineoplastic Cancer Research Cell Survival Cyclin A Medicine (miscellaneous) Apoptosis Flowers 03 medical and health sciences chemistry.chemical_compound Sapindaceae 0302 clinical medicine Cell Line Tumor Humans Propidium iodide Viability assay Cell Proliferation 030109 nutrition & dietetics Nutrition and Dietetics biology Plant Extracts Cell growth Cell Cycle Drug Synergism Cell cycle Molecular biology digestive system diseases Oncology chemistry Cell culture 030220 oncology & carcinogenesis biology.protein Trypan blue Fluorouracil Colorectal Neoplasms |
| Zdroj: | Nutrition and Cancer. 72:209-217 |
| ISSN: | 1532-7914 0163-5581 |
| DOI: | 10.1080/01635581.2019.1622739 |
| Popis: | To investigate the influence of longan flower extract (LFE) on the sensitization of colorectal cancer (CRC) cells to 5-fluorouracil (5-FU) treatment, HT-29, Colo 320DM and SW480 cells were treated with LFE and 5-FU alone and in combination, and the cell viability was then assessed by trypan blue exclusion, the cell cycle by propidium iodide staining, the mitochondria membrane potential by rhodamine 123 staining, and the expression levels of associated genes by immunoblotting and quantitative real-time polymerase chain reaction. LFE and 5-FU synergistically inhibited cell proliferation of HT-29 and Colo 320DM cells. Combined treatment also elevated the level of loss of mitochondria membrane potential of these two CRC cells and arrested HT-29 cells in the S phase of the cell cycle, in association with down-regulation of cyclin A mRNA expression. LFE synergistically potentiated chemosensitivity to 5-FU in at least two CRC cell lines. The results indicated that LFE has potential as a novel agent for the sensitization of CRC cells to 5-FU. |
| Databáze: | OpenAIRE |
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