| Autor: |
Grandt, Caine Lucas, Brackmann, Lara Kim, Poplawski, Alicia, Schwarz, Heike, Hummel-Bartenschlager, Willempje, Hankeln, Thomas, Kraemer, Christiane, Marini, Federico, Zahnreich, Sebastian, Schmitt, Iris, Drees, Philipp, Mirsch, Johanna, Grabow, Desiree, Schmidberger, Heinz, Binder, Harald, Hess, Moritz, Galetzka, Danuta, Marron, Manuela |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.21018958 |
| Popis: |
Additional file 3. Primer for and results of qRT-PCR. AF3a. Constructed and purchased primer sequences for the qPCR. AF3b. Log10 relative expression measured in quantitative polymerase reverse transcriptase chain reaction (qRT-PCR) for selected genes compared to TATA-Box Binding Protein (TBP), stratified by group (each n = 6, three replicates per individuals) and radiation dose. Significance stars imply the p-value thresholds of two-sided t-tests after adjustment for false discovery rate (* p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001). N0 = fibroblasts of cancer-free controls, N1 = fibroblasts of childhood-cancer survivors, N2+ = fibroblasts of childhood-cancer survivors with at least one second primary neoplasm. AF3c. Log10-relative expression measured in quantitative polymerase reverse transcriptase chain reaction (qRT-PCR) for mouse double minute 2 (MDM2) and Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) compared to TATA-Box Binding Protein (TBP). Data adapted from Brackmann et al. 2020, coloured by group (each n = 2, three replicates per individual) and stratified by radiation dose. Significance stars imply the p-value thresholds of two-sided t-tests (* p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001). N0 = fibroblasts of cancer-free controls, N1 = fibroblasts of childhood-cancer survivors, N2+ = fibroblasts of childhood-cancer survivors with at least one second primary neoplasm. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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