Mechanism of copper mediated triple helix formation at neutral pH in Drosophila satellite repeats
| Autor: | J. Lacoste, F. Geinguenaud, J. Liquier, Catherine Gouyette, C. Paris |
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| Přispěvatelé: | Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Models
Molecular inorganic chemicals Circular dichroism Pyrimidine Guanine Metal ions in aqueous solution Biophysics Infrared spectroscopy DNA Satellite 010402 general chemistry 01 natural sciences SEQUENCE CIRCULAR-DICHROISM FTIR SPECTROSCOPY 03 medical and health sciences chemistry.chemical_compound IR SPECTROSCOPY Nucleic Acids Animals Computer Simulation 030304 developmental biology 0303 health sciences IN-SITU DEOXYRIBONUCLEIC ACIDS DNA Hydrogen-Ion Concentration 0104 chemical sciences Crystallography Drosophila melanogaster Models Chemical chemistry Biochemistry METAL-IONS Nucleic Acid Conformation DNA STRUCTURES DEOXYOLIGONUCLEOTIDES POLYNUCLEOTIDIC STRUCTURES Copper Cytosine Microsatellite Repeats Triple helix |
| Zdroj: | Biophysical Journal Biophysical Journal, Biophysical Society, 2007, 92 (7), pp.2498-2506. ⟨10.1529/biophysj.106.099788⟩ |
| ISSN: | 0006-3495 1542-0086 |
| DOI: | 10.1529/biophysj.106.099788⟩ |
| Popis: | 1 - Article; The highly repeated Drosophila melanogaster AAGAGAG satellite sequence is present at each chromosome centromere of the fly. We demonstrate here how, under nearly physiological pH conditions, these sequences can form a pyrimidine triple helix containing T.A-T and C-Cu.G-C base triplets, stabilized by Cu2+ metal ions in amounts mirroring in vivo concentrations. Ultraviolet experiments were used to monitor the triple helix formation at pH 7.2 in presence of Cu2+ ions. Triplex melting is observed at 23 degrees C. Furthermore, a characteristic signature of triple helix formation was obtained by Fourier transform infrared spectroscopy. The stabilization of the C.G-C base triplets at pH7.2 is shown to occur via interactions of Cu2+ ions on the third strand cytosine N3 atom and on the guanine N7 atom of the polypurine target strand forming C-Cu.G-C triplets. Under the same neutral pH conditions in absence of Cu2+ ions, the triple helix fails to form. Possible biological implications are discussed. |
| Databáze: | OpenAIRE |
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