Non-enzymatic electrochemical cholesterol sensor based on strong host-guest interactions with a polymer of intrinsic microporosity (PIM) with DFT study

Autor: Hamideh Imanzadeh, Neil B. McKeown, Mina Ghiasi, Sabine Szunerits, Mandana Amiri, Negin Jahani, Frank Marken, Rabah Boukherroub
Přispěvatelé: University of Mohaghegh Ardabili, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), University of Bath [Bath], The authors received support of this work from the University ofMohaghegh Ardabili Research Council, Ardabil, Iran (grant number3051234502)., Alzahra University, Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), School of Chemistry, University of Edinburgh, University of Edinburgh, The authors received support of this work from the University of Mohaghegh Ardabili Research Council, Ardabil, Iran (grant number 3051234502).
Rok vydání: 2021
Předmět:
Zdroj: Jahani, N, Amiri, M, Ghiasi, M, Imanzadeh, H, Boukherroub, R, Szunerits, S, Marken, F & McKeown, N B 2021, ' Non-enzymatic electrochemical cholesterol sensor based on strong host-guest interactions with a polymer of intrinsic microporosity (PIM) with DFT study ', Analytical and bioanalytical chemistry, vol. 413, no. 26, pp. 6523-6533 . https://doi.org/10.1007/s00216-021-03616-w
Analytical and Bioanalytical Chemistry
Analytical and Bioanalytical Chemistry, Springer Verlag, 2021, 413 (26), pp.6523-6533. ⟨10.1007/s00216-021-03616-w⟩
Analytical and Bioanalytical Chemistry, 2021, 413 (26), pp.6523-6533. ⟨10.1007/s00216-021-03616-w⟩
ISSN: 1618-2650
1618-2642
Popis: Advances in materials science have accelerated the development of diagnostic tools with the last decade witnessing the development of enzyme-free sensors, owing to the improved stability, low cost and simple fabrication of component materials. However, the specificity of non-enzymatic sensors for certain analytes still represents a challenging task, for example the determination of cholesterol level in blood is vital due to its medical relevance. In this work, a reagent displacement assay for cholesterol sensing in serum samples was developed. It is based on coating of a glassy carbon electrode with a polymer of intrinsic microporosity (PIM) that forms a host-guest complex with methylene blue (MB). In the presence of cholesterol, the MB electroactive probe was displaced due to the stronger association of cholesterol guest to the PIM host. The decrease in the oxidative current was proportional to the cholesterol concentration achieving a detection limit of approximately 0.1 nM. Moreover, to further assist the experimental studies, comprehensive theoretical calculations are also performed by using density functional theory (DFT) calculations. Graphical abstract: [Figure not available: see fulltext.].
Databáze: OpenAIRE
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