Interpretation of immunoblots for Lyme borreliosis using a semiquantitative approach
| Autor: | Luc Binet, Eric Dayer, Karine Ryffel, O Péter |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
neuroborreliosis
Microbiology (medical) Pathology medicine.medical_specialty Population specificity Spirochaetaceae immunoblots Lyme Arthritis medicine.disease_cause Lyme disease Borrelia medicine Borrelia burgdorferi education erythema migrans Lyme borreliosis education.field_of_study biology Acrodermatitis Borrelia garinii General Medicine biology.organism_classification medicine.disease bacterial infections and mycoses sensitivity Infectious Diseases arthritis Immunology Neuroborreliosis |
| Zdroj: | Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. 4(4) |
| ISSN: | 1469-0691 |
| Popis: | OBJECTIVE: To test the performances of new Borrelia garinii immunoblots specific for Borrelia burgdorferi sensu lato with a selected panel of sera from patients with various clinical presentations of Lyme borreliosis. METHODS: In order to establish the sensitivity and the specificity of these immunoblots, we tested serum samples obtained from patients with early- and late-stage Lyme disease (erythema migrans n=35, neuroborreliosis n=61, acrodermatitis chronica atrophicans (ACA) n=27 and arthritis n=41), from patients with diagnoses and laboratory findings associated with serologic cross-reactivity to Lyme disease (syphilis n=12, Epstein-Barr infection n=9, autoimmune markers n=29) and from blood donors residing in regions of low and medium endemicity (n=80, n=100). RESULTS: The combined sensitivity (IgG and IgM) of the tests was 90% for patients with erythema migrans, 92% for neuroborreliosis, 96% for ACA and 100% for Lyme arthritis. The specificity of the IgG immunoblot was 94%, and that of the IgM immunoblot was 97%, taking into account the prevalence of borrelia antibodies in the overall population. Interpretation of these immunoblots is based on scores allocated to different specific borrelia antigens. CONCLUSIONS: The Western blot technology is extremely useful in dissecting the immune response to borrelia infections, which develops gradually over a period of weeks to years and which involves the appearance of IgM and IgG antibodies directed against a number of borrelia-associated proteins. |
| Databáze: | OpenAIRE |
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