SGS1 is required for telomere elongation in the absence of telomerase
| Autor: | Edward J. Louis, Ian D. Hickson, Darren Lester, Pei-Hsiu Huang, Rhona H. Borts, Fiona E. Pryde, Rachelle L Maddison |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Telomere Recombination
Telomerase Saccharomyces cerevisiae Proteins Cell Survival Saccharomyces cerevisiae General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Telomerase RNA component 0302 clinical medicine Telomerase reverse transcriptase 030304 developmental biology Telomere-binding protein 0303 health sciences Agricultural and Biological Sciences(all) biology RecQ Helicases Biochemistry Genetics and Molecular Biology(all) DNA Helicases Helicase Telomere Molecular biology enzymes and coenzymes (carbohydrates) 030220 oncology & carcinogenesis Mutation biology.protein General Agricultural and Biological Sciences Sgs1 |
| Zdroj: | Current biology : CB. 11(2) |
| ISSN: | 0960-9822 |
| Popis: | In S. cerevisiae , mutations in genes that encode telomerase components, such as the genes EST1 , EST2 , EST3, and TLC1, result in the loss of telomerase activity in vivo [1–3]. Two telomerase-independent mechanisms can overcome the resulting senescence. Type I survival is characterized by amplification of the subtelomeric Y′ elements with a short telomere repeat tract at the terminus [4]. Type II survivors arise through the abrupt addition of long tracts of telomere repeats [4–6]. Both mechanisms are dependent on RAD52 [4, 5] and on either RAD50 or RAD51 [6, 7]. We show here that the telomere elongation pathway in yeast (type II) is dependent on SGS1 , the yeast homolog of the gene products of Werner's ( WRN ) [8] and Bloom's ( BLM ) [9] syndromes. Survival in the absence of SGS1 and EST2 is dependent upon RAD52 and RAD51 but not RAD50 . We propose that the RecQ family helicases are required for processing a DNA structure specific to eroding telomeres. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|