A novel hairpin-like antimicrobial peptide from barnyard grass (Echinochloa crusgalli L.) seeds: Structure-functional and molecular-genetics characterization
| Autor: | Tatiana V. Dimitrieva, Dmitry Y. Ryazantsev, Polina E. Drobyazina, N. V. Khadeeva, Sergey K. Zavriev, Eugene A. Rogozhin, Tsezi A. Egorov, Eugene V. Grishin |
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| Rok vydání: | 2013 |
| Předmět: |
Antifungal Agents
Pseudogene Molecular Sequence Data Pseudomonas syringae Peptide Microbial Sensitivity Tests Biology Biochemistry Homology (biology) Protein Structure Secondary law.invention Inhibitory Concentration 50 Fusarium law Actinomycetales Gene family Plant Immunity Amino Acid Sequence Gene Plant Diseases Plant Proteins chemistry.chemical_classification Sequence Homology Amino Acid Plant Extracts food and beverages General Medicine biology.organism_classification Anti-Bacterial Agents Pectobacterium carotovorum chemistry Echinochloa Seeds Recombinant DNA Aspergillus niger Antibacterial activity Bacteria Antimicrobial Cationic Peptides |
| Zdroj: | Biochimie. 99 |
| ISSN: | 1638-6183 |
| Popis: | A novel plant hairpin-like defense polypeptide named EcAMP3 was isolated from latent barnyard grass (Echinochloa crusgalli L.) seeds. The native peptide and its recombinant analogue were characterized. EcAMP3 displays antifungal and antibacterial activity in vitro. The gene family encoding EcAMPs precursor protein was also characterized; the genes and pseudogenes of this family show 97–100% homology. Every member of EcAMPs precursor family contains seven identical cysteine motifs: C1XXXC2(11–13)C3XXXC4. One of those motifs corresponds to the isolated peptide. EcAMP3 is the first member of the plant hairpin-like peptide family that inhibits the growth of phytopathogenic bacteria. Obtained results can explain the nature of the complex resistance of barnyard grass to a variety of pathogenic microorganisms. |
| Databáze: | OpenAIRE |
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