INTRAVENOUS ANESTHESIA INHIBITS LEUKOCYTE-ENDOTHELIAL INTERACTIONS AND EXPRESSION OF CD11B AFTER HEMORRHAGE
| Autor: | Charles S. Reilly, Nicola J. Brown, Zoë L. S. Brookes, Bethan K. Lawton |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Time Factors Hemorrhage CD18 Pharmacology Critical Care and Intensive Care Medicine chemistry.chemical_compound Integrin complex Cell Adhesion Leukocytes medicine Animals Ketamine Rats Wistar Thiopental Propofol Inflammation CD11b Antigen Platelet-activating factor business.industry Endothelial Cells Rats Gene Expression Regulation Intravenous anesthesia chemistry Anesthesia Anesthetic Anesthesia Intravenous Emergency Medicine Endothelium Vascular business Intravital microscopy medicine.drug |
| Zdroj: | Shock. 25:492-499 |
| ISSN: | 1073-2322 |
| DOI: | 10.1097/01.shk.0000209541.76305.8e |
| Popis: | UNLABELLED: Hemorrhage increases adhesion of leukocytes to the venular endothelium, mediated by increased expression of the Mac-1 integrin complex (CD18/CD11b) present on leukocytes. Anesthetic agents may possess anti-inflammatory properties. Hence, this study determined the effects of i.v. anesthesia on leukocyte adhesion after hemorrhage in relation to expression of CD11b. METHODS: Male Wistar rats were (n = 57) anesthetized i.v. with propofol (Diprivan) and fentanyl, ketamine, or thiopental. During anesthesia, 10% of total blood volume was removed and intravital microscopy used to observe the rat mesentery and measure leukocyte (neutrophils) rolling and adhesion in postcapillary venules (15 - 25 microm). Flow cytometry was also used to determine CD11b expression on neutrophils from blood removed at the end of these experiments (n = 25) or blood incubated with anesthetic agents and activated with platelet activating factor ex vivo (0.1 micromol/L) (n = 24). RESULTS: Hemorrhage increased leukocyte adhesion (stationary count per 150 microm) in rats anesthetized with thiopental (baseline, 3.4 +/- 1.2; hemorrhage, 6.7 +/- 2.0; P < 0.05) but not in those receiving either ketamine (baseline, 3.6 +/- 1.3; hemorrhage, 3.3 +/- 1.3) or propofol/fentanyl (baseline, 6.2 +/- 2.0; hemorrhage, 5.8 +/- 0.8). Neutrophils collected from thiopental-treated rats had elevated CD11b expression with thiopental (mean fluorescence baseline, 67.5 +/- 1.3; hemorrhage, 83.6 +/- 5.3; P < 0.05) but not with propofol/fentanyl (mean fluorescence baseline, 69.1 +/- 1.3; hemorrhage, 65.9 +/- 1.6), and ketamine-treated rats (mean fluorescence baseline, 74.3 +/- 2.1; hemorrhage, 74.8 +/- 1.1). Ketamine also inhibited upregulation of CD11b with platelet activating factor ex vivo. CONCLUSIONS: After hemorrhage, leukocyte adhesion and CD11b expression increased during thiopental anesthesia, but propofol/fentanyl and ketamine protected against hemorrhage-induced leukocyte adhesion. The anti-inflammatory effect of ketamine was mediated by direct inhibition of CD11b expression on leukocytes. |
| Databáze: | OpenAIRE |
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