Anti-idiotypic antibodies to bovine herpesvirus-1 inhibit virus infection in cell cultures
| Autor: | D.J. Orten, Frank Blecha, W. Xue, O. Y. AbdelMagid, H.C. Minocha |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
biology
medicine.drug_class viruses Antibodies Monoclonal General Medicine Antibodies Viral Monoclonal antibody biology.organism_classification Virology Molecular biology Epitope Virus Bovine herpesvirus 1 Antibodies Anti-Idiotypic Cell Line Antigen Affinity chromatography medicine biology.protein Animals Simplexvirus Binding site Antibody |
| Zdroj: | Archives of Virology. 122:163-173 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | A panel of murine monoclonal antibodies (MAbs) to bovine herpesvirus-1 (BHV-1) was prepared. Three of them were neutralizing MAbs and reacted against 130/75/50 kDa, 77 kDa, or 97 kDa glycoproteins (gp). A fourth non-neutralizing MAb recognized the 97 kDa gp. Competition radioimmunoassay demonstrated that each of the four MAbs reacted against a different virus epitope. Anti-idiotypic antibodies (anti-id) to the four MAbs were produced in rabbits and purified by sequential immunoaffinity chromatography. Each anti-id inhibited the binding of its respective MAb to BHV-1 in competitive ELISA and blocked BHV-1 neutralizing activity of the MAb. This inhibition suggested that the anti-ids were specific for the antigen binding site of the MAbs. Treatment of MDBK cells with anti-ids inhibited BHV-1 infection, which suggested that the anti-ids block a cellular component essential for virus infection. Absence of significant cross-reactivity among the anti-ids for heterologous MAbs indicated that they recognized unique determinants on the antigen binding site of the homologous MAb. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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