EMAP-II expression is associated with macrophage accumulation in primary uveal melanoma
| Autor: | Dirk J. Ruiter, Lia Schalkwijk, Ruud Clarijs, Robert M.W. de Waal |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Uveal Neoplasms
Pathology medicine.medical_specialty Intercellular Adhesion Molecule-1 Vascular Endothelial Growth Factor C Vascular Cell Adhesion Molecule-1 Apoptosis Cell Count Endothelial Growth Factors Immunoenzyme Techniques chemistry.chemical_compound Von Willebrand factor Cell Movement von Willebrand Factor medicine Humans Melanoma biology Neovascularization Pathologic Macrophages RNA-Binding Proteins Chemotaxis Uvea medicine.disease Vascular Endothelial Growth Factor Receptor-2 Neoplasm Proteins Tumor microenvironment [UMCN 1.3] Vascular endothelial growth factor medicine.anatomical_structure Vascular endothelial growth factor C chemistry biology.protein Cancer research Immunohistochemistry Cytokines Endothelium Vascular |
| Zdroj: | Investigative Ophthalmology and Visual Science, 44, 1801-6 Investigative Ophthalmology and Visual Science, 44, 5, pp. 1801-6 |
| ISSN: | 0146-0404 |
| Popis: | Contains fulltext : 144480.pdf (Publisher’s version ) (Open Access) PURPOSE: Primary uveal melanoma may contain arcs, loops, and networks of periodic acid-Schiff (PAS)-positive patterns, along which numerous macrophages are present. Their recruitment into tumor tissue is mediated by chemotactic cytokines, for which vascular endothelial growth factor (VEGF)-C and endothelial monocyte-activating polypeptide ((EMAP)-II are candidates. In this study, the extent of VEGF-C and EMAP-II immunoreaction was related to infiltration of macrophages. METHODS: Serial sections of 25 primary uveal melanoma lesions were analyzed by immunohistochemistry. RESULTS: The analysis showed no correlation of VEGF-C immunoreaction and localization of macrophages. However, accumulation of macrophages occurred at sites of EMAP-II expression, especially in areas containing nests of tumor cells, surrounded by arcs, loops, and network patterns. In tumors with a strong EMAP-II immunoreaction, the adhesion molecule intracellular adhesion molecule (ICAM)-1 was strongly expressed on endothelial cells. EMAP-II-positive endothelial cells did not express VEGF receptor-2. However, extensive release of von Willebrand factor was observed. Signs of apoptosis were found neither in tumor cells nor endothelial cells. CONCLUSIONS: In uveal melanoma, macrophages accumulate at sites of EMAP-II expression. Based on the results, it may be hypothesized that this process of chemotaxis is facilitated by EMAP-II-dependent expression of ICAM-1 on vascular endothelial cells and concomitantly leads to localized vascular damage, as indicated by release of von Willebrand factor. |
| Databáze: | OpenAIRE |
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