Comparative genomic analysis between newly sequenced Brucella suis Vaccine Strain S2 and the Virulent Brucella suis Strain 1330
| Autor: | Xiang Mao, Wen Zhang, Yao Yu, Li-Li Tian, Qi Zhong, Hai Jiang, Bo Ni, Dong dong Di, Xin ping Yi, Jing li Kang, Wei xing Fan, You yu He, Lin Xia, Feng Ye, Bu yun Cui |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Brucella suis Biovar 030106 microbiology Brucella Vaccine Virulence Brucella suis vaccine strain S2 Brucella Polymorphism Single Nucleotide Microbiology 03 medical and health sciences Gene Order Genetics Comparative genomic analysis Whole genome sequencing Comparative Genomic Hybridization Bacterial disease biology Computational Biology High-Throughput Nucleotide Sequencing Genomics Periplasmic space Chromosomes Bacterial biology.organism_classification Brucella suis virulent strain 1330 Virology Genes Bacterial Genome sequence Bacterial outer membrane Genome Bacterial Research Article Biotechnology |
| Zdroj: | BMC Genomics |
| ISSN: | 1471-2164 |
| Popis: | Background Brucellosis is a bacterial disease caused by Brucella infection. In the late fifties, Brucella suis vaccine strain S2 with reduced virulence was obtained by serial transfer of a virulent B. suis biovar 1 strain in China. It has been widely used for vaccination in China since 1971. Until now, the mechanisms underlie virulence attenuation of S2 are still unknown. Results In this paper, the whole genome sequencing of S2 was carried out by Illumina Hiseq2000 sequencing method. We further performed the comparative genomic analysis to find out the differences between S2 and the virulent Brucella suis strain 1330. We found premature stops in outer membrane autotransporter omaA and eryD genes. Single mutations were found in phosphatidylcholine synthase, phosphorglucosamine mutase, pyruvate kinase and FliF, which have been reported to be related to the virulence of Brucella or other bacteria. Of the other different proteins between S2 and 1330, such as Omp2b, periplasmic sugar-binding protein, and oligopeptide ABC transporter, no definitive implications related to bacterial virulence were found, which await further investigation. Conclusions The data presented here provided the rational basis for designing Brucella vaccines that could be used in other strains. Electronic supplementary material The online version of this article (doi:10.1186/s12864-016-3076-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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