The influence of experimentally induced endometritis on the PPAR expression profile in the bovine endometrium
| Autor: | M. Łupicka, A.A. Szczepańska, Anna Korzekwa, P. Łada, B.M. Socha |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Peroxisome Proliferator-Activated Receptors Peroxisome proliferator-activated receptor Cattle Diseases Stimulation Endometrium Andrology 03 medical and health sciences Food Animals medicine Escherichia coli Animals RNA Messenger Small Animals Receptor chemistry.chemical_classification Messenger RNA Equine medicine.disease 030104 developmental biology medicine.anatomical_structure chemistry Nuclear receptor Animal Science and Zoology Cattle Female Endometritis Signal transduction Transcriptome |
| Zdroj: | Theriogenology. 122 |
| ISSN: | 1879-3231 |
| Popis: | Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors of the nuclear receptor superfamily. The PPARs activity can be modified e.g. by arachidonic acid metabolites. Escherichia coli (E. coli) is one of the main infectious agent of endometritis in dairy cows. We hypothesized that PPAR expression profile change in the bovine endometrium under the influence of LPS or E. coli. In experiment 1, endometrial explants were obtained post mortem from heifers and incubated without (control) or with LPS for 12, 24, 48, 72 and 96 h. In experiment 2, heifers were intrauterine infused with 0.9% NaCl (control) or with E. coli suspension in 0.9% NaCl. Endometrial biopsies were performed before (0 h) and 12, 24, 48, 72, 96 h after the infusions. In experiment 1, the increase in protein expression was observed for PPARα 48 h, for PPARβ/δ 24, 72 and 96 h, whereas for PPARγ 12, 24 and 96 h after LPS treatment relative to the control groups. In experiment 2, the up-regulation in protein expression was observed for PPARα 48 and 72 h, for PPARβ/δ 72 and 96 h, for PPARγ1 and PPARγ2 12 and 96 h after the intrauterine infusion with E. coli suspension compared to the control group. Changes in mRNA and protein PPAR expression profile in endometrial explants under the exposure of LPS indicate participation of these nuclear receptors in signal transduction during stimulation with LPS. The patterns of mRNA and protein PPAR expression in endometrial bioptates suggest that during experimentally induced endometritis in vivo, PPARs role may be connected both with enhancement of inflammation as well restoring physiological conditions in uterus. |
| Databáze: | OpenAIRE |
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