Calvaria derived osteogenic cells: phenotypic expression in culture
| Autor: | Esther Berger, Nahum Fine, Arieh Harell, Zvi Shimshoni, Dalia Somjen, Itzhak Binderman |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Stimulation
Matrix (biology) Biochemistry Rheumatology Physical Stimulation medicine Animals Orthopedics and Sports Medicine Bone Resorption Molecular Biology Cells Cultured Bone Development Osteoblasts DNA synthesis biology Chemistry Skull Osteoblast Cell Biology Adaptation Physiological Rats medicine.anatomical_structure Phenotype Collagenase Osteocalcin biology.protein Alkaline phosphatase Stress Mechanical medicine.drug Hormone |
| Zdroj: | Connective tissue research. 20(1-4) |
| ISSN: | 0300-8207 |
| Popis: | The osteoblast phenotype is characterized by its ability to (a) synthesize a well defined mineralized collage nous matrix, (b) regulate the remodeling process by synthesizing local hormones (PGE2) and specific molecules (osteocalcin) and enzymes (alkaline phosphatase and collagenase), (c) respond to a variety of hormones (PTH, PGs, vitamin-D metabolites, steroids and growth factors), (d) respond to mechanical stimulation. Most of osteoblast culture systems meet many of the above qualifications though most fail to show the PTH effect on DNA synthesis, (c), and mechanical stimulation, (d). Here we show that by using trypsin digestion and serum-containing low calcium medium (0.25 mM), all the above listed osteoblast phenotypic characteristics are demonstrated including their responsiveness to mechanical stimulation and the PTH effect on DNA synthesis. |
| Databáze: | OpenAIRE |
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