Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae
| Autor: | Damian P. Drew, Ye Zhang, Corinna Weitzel, Christoph Wilhelm Sensen, Henrik Toft Simonsen, Bjørn Dueholm |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Thapsia laciniata guaianolides thapsane Apiaceae sesquiterpenoids 01 natural sciences Polymerase Chain Reaction lcsh:Chemistry Terpene Transcriptome chemistry.chemical_compound Cytochrome P-450 Enzyme System Gene Expression Regulation Plant lcsh:QH301-705.5 Spectroscopy Phylogeny Thapsia Plant Proteins Electrophoresis Agar Gel 0303 health sciences Guaiene food and beverages Geranyltranstransferase General Medicine Biodiversity 3. Good health Computer Science Applications Thapsia (plant) Multigene Family Sesquiterpenes Citric Acid Cycle Molecular Sequence Data Artemisia annua Biology Sesquiterpene Genes Plant Catalysis Article Inorganic Chemistry 03 medical and health sciences Botany Amino Acid Sequence Physical and Theoretical Chemistry Molecular Biology 030304 developmental biology Alkyl and Aryl Transferases Sequence Analysis RNA Terpenes Gene Expression Profiling Organic Chemistry Aldehyde Dehydrogenase biology.organism_classification Terpenoid lcsh:Biology (General) lcsh:QD1-999 chemistry Sequence Alignment 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 14; Issue 5; Pages: 9080-9098 International Journal of Molecular Sciences, Vol 14, Iss 5, Pp 9080-9098 (2013) |
| ISSN: | 1422-0067 |
| Popis: | Thapsia laciniata Rouy (Apiaceae) produces irregular and regular sesquiterpenoids with thapsane and guaiene carbon skeletons, as found in other Apiaceae species. A transcriptomic analysis utilizing Illumina next-generation sequencing enabled the identification of novel genes involved in the biosynthesis of terpenoids in Thapsia. From 66.78 million HQ paired-end reads obtained from T. laciniata roots, 64.58 million were assembled into 76,565 contigs (N50: 1261 bp). Seventeen contigs were annotated as terpene synthases and five of these were predicted to be sesquiterpene synthases. Of the 67 contigs annotated as cytochromes P450, 18 of these are part of the CYP71 clade that primarily performs hydroxylations of specialized metabolites. Three contigs annotated as aldehyde dehydrogenases grouped phylogenetically with the characterized ALDH1 from Artemisia annua and three contigs annotated as alcohol dehydrogenases grouped with the recently described ADH1 from A. annua. ALDH1 and ADH1 were characterized as part of the artemisinin biosynthesis. We have produced a comprehensive EST dataset for T. laciniata roots, which contains a large sample of the T. laciniata transcriptome. These transcriptome data provide the foundation for future research into the molecular basis for terpenoid biosynthesis in Thapsia and on the evolution of terpenoids in Apiaceae. |
| Databáze: | OpenAIRE |
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