Cigarette smoking induces overexpression of hepatocyte growth factor in type II pneumocytes and lung cancer cells
| Autor: | Kuan-Chih Chow, Tze-Yi Lin, Hsuan Hua Huang, Chih Yi Chen, Tzu Lin Chuang, Torng Sen Lin, Hong Chen Chen, Jin Tang Chen, Shu-Fen Chiang, Shiow-Her Chiou |
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| Rok vydání: | 2005 |
| Předmět: |
Pulmonary and Respiratory Medicine
Lung Neoplasms Clinical Biochemistry In situ hybridization Biology Adenocarcinoma Proto-Oncogene Mas Mice Carcinoma Non-Small-Cell Lung Cell Line Tumor Gene expression medicine Animals Humans Lung cancer Molecular Biology In Situ Hybridization Oligonucleotide Array Sequence Analysis Hepatocyte Growth Factor Smoking Epithelial Cells Cell Biology respiratory system Proto-Oncogene Proteins c-met medicine.disease Molecular biology Gene Expression Regulation Neoplastic Pulmonary Alveoli Suppression subtractive hybridization Cell culture Immunohistochemistry Hepatocyte growth factor medicine.drug |
| Zdroj: | American journal of respiratory cell and molecular biology. 34(3) |
| ISSN: | 1044-1549 |
| Popis: | We examined gene expression of hepatocyte growth factor (HGF) and HGF receptor (HGFR), or product of proto-oncogene c-met (c-met), in smokers and nonsmokers with adenocarcinoma (ADC) by suppression subtractive hybridization and microarray techniques. Expression of HGF and c-met was confirmed by RT-PCR. HGF content in the respective tumor mass and nontumor lung tissue was measured by ELISA. HGF in pathologic samples was localized by immunohistochemistry and in situ hybridization. Our results indicate that overexpression of HGFR was frequently detected in ADC cells, whereas overexpression of HGF was detected in alveolar type II (ATII) cells. Overexpression of HGF was correlated with cigarette smoking and tumor stages. In vitro, HGF expression was evaluated in isolated murine ATII cells and in 12 ADC cell lines, and we found that nicotine activated HGF expression in ATII cells and lung cancer cells. |
| Databáze: | OpenAIRE |
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