Potential application of notochordal cells for intervertebral disc regeneration: an in vitro assessment
| Autor: | E Esther Potier, Keita Keita Ito, van M Marina Doeselaar, de Sah Stefan Vries |
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| Přispěvatelé: | Bioingénierie et Bioimagerie Ostéo-articulaires, Biomécanique et Biomatériaux Ostéo-Articulaires (B2OA (UMR_7052)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA), Department of Biomedical Engineering [Eindhoven], Eindhoven University of Technology [Eindhoven] (TU/e), University Medical Center [Utrecht], This work was supported by AOSpine International through an AOSpine Research Network grant (SRN2011_11)., Orthopaedic Biomechanics, École nationale vétérinaire d'Alfort (ENVA)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2014 |
| Předmět: |
lcsh:Diseases of the musculoskeletal system
Stromal cell lcsh:Surgery Notochord MESH: Intervertebral Disc Matrix (biology) Mesenchymal Stem Cell Transplantation nucleus pulposus cells 03 medical and health sciences 0302 clinical medicine marrow stromal cells [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] medicine Animals Humans Regeneration Cells Cultured Aggrecan 030304 developmental biology Extracellular Matrix Proteins 0303 health sciences Chemistry Regeneration (biology) MESH: Notochord MESH: Regeneration cell communication Intervertebral disc lcsh:RD1-811 In vitro Cell biology notochordal cells MESH: Cattle Collagen type I alpha 1 medicine.anatomical_structure Cattle Bone marrow lcsh:RC925-935 cell therapy Chondrogenesis 030217 neurology & neurosurgery bone marrow stromal cells MESH: Cells Cultured Biomedical engineering |
| Zdroj: | eCells and Materials Journal eCells and Materials Journal, AO Research Institute Davos, 2014, 28, pp.68-81. ⟨10.22203/eCM.v028a06⟩ European Cells & Materials, Vol 28, Pp 68-81 (2014) European Cells and Materials, 28, 68-81. Swiss Society for Biomaterials |
| ISSN: | 1473-2262 |
| DOI: | 10.22203/ecm.v028a06 |
| Popis: | With kind permission of full reproduction from eCM journal (www.ecmjournal.org). Founded by scientists for the benefit of Science rather than profit.; International audience; Recent studies suggest that notochordal cells (NCs) might be involved in intervertebral disc homeostasis, a role exploitable to counteract matrix degradation as observed during degeneration. This study aimed to evaluate the potential of NCs to promote matrix production by nucleus pulposus cells (NPCs) and to compare it to the currently proposed addition of bone marrow stromal cells (BMSCs). Using alginate beads, bovine NPCs were exposed for 28 d to porcine NC conditioned medium (NCCM); direct co-culture with porcine NCs or bovine BMSCs; or the combination of BMSCs and NCCM. Effects on cell proliferation, disc matrix production (proteoglycans, collagens) and disc matrix protein expression (aggrecan, collagen 1 and 2, SOX9) were determined and compared to TGFβ stimulation. NCCM strongly promoted NPC proliferation (x 2.2) and matrix production (x 3.9) to levels similar to that with TGFβ, whereas the direct addition of NCs had no effect. Co-culture of NPCs and BMSCs led to proteoglycan synthesis similar to NPCs alone, which was slightly improved by NCCM (x 1.5). Histological analysis confirmed biochemical data. Gene expression of analysed proteins remained stable for all groups and unaffected by medium conditions. NCs could substantially stimulate NPCs through factors secreted into conditioned medium and in levels similar to the addition of BMSCs. This study showed that molecular agents secreted by NCs constitute a promising alternative to the proposed "standard" injection of BMSCs for disc repair: their effects are similar, do not require the injection of a large number of cells and can be further amplified when the factors are identified. |
| Databáze: | OpenAIRE |
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