Impaired clonogenic growth of myelodysplastic bone marrow progenitors in vitro is irrelevant to their apoptotic state
| Autor: | Alexandra Kouraklis-Symeonidis, Theodora Kakagianni, Ilina Micheva, Sotiria Michalopoulou, Nicholas C. Zoumbos, Argiris Symeonidis |
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| Rok vydání: | 2003 |
| Předmět: |
Adult
Male Cancer Research Programmed cell death Chronic myelomonocytic leukemia Stem cell factor Antigens CD34 Apoptosis Bone Marrow Cells Biology In Vitro Techniques hemic and lymphatic diseases medicine Humans Progenitor cell Annexin A5 Clonogenic assay Cells Cultured Tumor Stem Cell Assay Aged Ineffective Hematopoiesis Aged 80 and over Stem Cells Hematology Middle Aged medicine.disease Hematopoiesis medicine.anatomical_structure Oncology Myelodysplastic Syndromes Immunology Cancer research Female Bone marrow Refractory anemia with excess of blasts |
| Zdroj: | Leukemia research. 28(8) |
| ISSN: | 0145-2126 |
| Popis: | Excessive intramedullary apoptosis has been considered to account for the paradox of hypercellular marrow and refractory cytopenias in myelodysplastic syndrome (MDS). However, a causative relationship of apoptosis to the progenitor’s defective clonogenic growth has not been sufficiently demonstrated. We investigated the degree of apoptosis and its contribution to ineffective hematopoiesis in MDS, by assessing the differential clonogenic capacity of purified “apoptotic” and “non-apoptotic” bone marrow progenitors in a short-term semisolid culture system. Although increased apoptosis was indeed detected in MDS bone marrow progenitors, there was no correlation between the existence of apoptosis and culture performance. Non-apoptotic as well as apoptotic CD34 + cells gave similar patterns of growth, both defective compared to normal. The ability of “apoptotic” CD34 + cells to proceed in colony formation as well as the abnormal growth of “non-apoptotic” progenitors are probably pointing towards the need to reconsider the role of apoptosis in the defective clonogenicity of MDS. © 2004 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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