Immunogold localization of glutamine synthetase in soybean leaves, roots, and nodules

Autor: J. Brangeon, A. Forchioni, B. Hirel
Přispěvatelé: Unité de recherche Nutrition Azotée des Plantes (URNAP), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration
Rok vydání: 1989
Předmět:
Zdroj: Protoplasma
Protoplasma, Springer Verlag, 1989, 151, pp.88-97
ISSN: 1615-6102
0033-183X
DOI: 10.1007/bf01403445
Popis: Immunogold labelling was used to detect the cellular and sub-cellular distribution of glutamine synthetase (GS) in nodulatedGlycine max var. maple arrow. The protein was detected in thin sections of tissue embedded in LR white acrylic resin by employing two polyclonal antibody preparations, one active chloroplastic GS, the other against the cytosolic form of the enzyme. In the mature leaf tissue, GS was visualized only in the chloroplasts, exclusively within the stroma matrix; in the root cortical tissue, the enzyme was distributed homogenously in the cytosol but with a slight preferential localization associated with certain endomembranes, whereas in the root nodules both cytosolic and plastidial compartments were labelled in infected and uninfected cells. Particular to the infected cells, the bacteroids' inner matrix reacted slightly to the GS antibody and a strong signal was preferentially localized on the bacteroids' outer envelope membranes. In general, GS was more concentrated in nodules as estimated by gold particle distribution, whether in the cytosol, plastids or on the bacteroid envelope membranes, than in either root tissue or leaf tissue. Although the cytoplasmic labelling density in nodules was similar in uninfected and infected cells, certain structural features in the latter (abundant cytosol, numerous GS-positive bacteroids and GS-reactive proplastids) contribute to a more enzyme-rich type than its uninfected counterpart.
Databáze: OpenAIRE
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