Self-collected and clinician-collected anal swabs show modest agreement for HPV genotyping
| Autor: | Racheal S. Dube Mandishora, Joel M. Palefsky, Megan B. Fitzpatrick, Trine B. Rounge, Zvavahera M. Chirenje, Babill Stray-Pedersen, Irene Kraus Christiansen, Massimo Tommasino, Sonja Lagström, Ole Herman Ambur |
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| Přispěvatelé: | Scheurer, Michael |
| Rok vydání: | 2021 |
| Předmět: |
Cross-sectional study
Anal Canal Pathology and Laboratory Medicine Medical Conditions 0302 clinical medicine Immunodeficiency Viruses 80 and over Early Detection of Cancer Cancer Aged 80 and over Coinfection virus diseases Anus Neoplasms Oncology Medical Microbiology Viral Pathogens Medicine HIV/AIDS Human Papillomavirus Infection medicine.medical_specialty Genotyping Genotype Science Sexually Transmitted Diseases Gastroenterology and Hepatology Microbiology Specimen Handling 03 medical and health sciences Cancer Detection and Diagnosis Genetics Anal cancer Humans Molecular Biology Techniques Microbial Pathogens Molecular Biology Aged AIDS-Related Opportunistic Infections Genitourinary Infections Organisms Human Papillomavirus DNA medicine.disease 030104 developmental biology Cross-Sectional Studies Africa DNA Viral Sexually Transmitted Infections Digestive Diseases RNA viruses 0301 basic medicine Viral Diseases Cervical Cancer Geographical Locations Medicine and Health Sciences Mass Screening 030212 general & internal medicine Viral Papillomaviridae Cervical cancer Multidisciplinary Middle Aged Infectious Diseases Viruses Female Pathogens Cervicography Cancer Screening Research Article Adult Zimbabwe Human papillomavirus Papillomaviruses Adolescent General Science & Technology Urology Anal and Rectal Disorders Research and Analysis Methods Young Adult McNemar's test Diagnostic Medicine Internal medicine Retroviruses medicine Biology and life sciences business.industry Lentivirus Papillomavirus Infections Cancers and Neoplasms HIV Good Health and Well Being Anal Cancer People and Places Human papillomavirus infections DNA viruses business Gynecological Tumors |
| Zdroj: | PloS one, vol 16, iss 4 PLoS ONE, Vol 16, Iss 4, p e0250426 (2021) e0250426 PLOS ONE PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Background & aimWomen with HIV/HPV coinfection and cervical lesions are at increased risk of developing HPV related anal cancer. Self-collection of anal swabs may facilitate HPV molecular testing in anal cancer screening, especially in high-risk groups, and yet it is not adequately studied. We evaluated level of agreement between self-collected anal swabs (SCAS) and clinician-collected anal swabs (CCAS) when used for HPV genotyping. We also described the anal HPV genotype distribution and HIV/HPV coinfection.MethodsWe performed a cross sectional study with participants from a visual-inspection-with-acetic-acid and cervicography (VIAC) clinic, in Harare, Zimbabwe. In a clinic setting, the women aged ≥18 years provided anal swabs in duplicate; first CCAS and then SCAS immediately after. HPV detection and genotyping were performed using next generation amplicon sequencing of a 450bp region of the HPV L1 gene. Level of agreement of HPV genotypes between CCAS and SCAS was calculated using the kappa statistic. McNemar tests were used to evaluate agreement in the proportion of genotypes detected by either method.ResultsThree-hundred women provided 600 samples for HPV genotyping. HPV genotypes were detected in 25% of SCAS and in 22% of CCAS. The most common genotypes with CCAS were HPV52, HPV62 and HPV70 and with SCAS were HPV62, HPV44, HPV52, HPV53 and HPV68. Total HPV genotypes detected in CCAS were more than those detected in SCAS, 32 versus 27. The agreement of HPV genotypes between the two methods was 0.55 in kappa value (k). The test of proportions using McNemar gave a Chi-square value of 0.75 (p = 0.39). Multiple HPV infections were detected in 28/75 and 29/67 women for CCAS and SCAS respectively.ConclusionsSCAS and CCAS anal swabs showed moderate agreement, with no statistically significant difference in the proportion of genotypes detected by either methods. Although the differences between the two methods were not statistically significant, CCAS detected more HPV genotypes than SCAS and more HPV infections were detected in SCAS than in CCAS. Our data suggest that self-collected anal swabs can be used as an alternative to clinician-collected anal swabs for HPV genotyping. |
| Databáze: | OpenAIRE |
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