Specification of basal region identity after asymmetric zygotic division requires mitogen-activated protein kinase 6 in rice
| Autor: | Kiyoe Ishimoto, Shino Sohonahra, Mitsuko Kaboshi-Kishi, Jun-ichi Itoh, Ken-ichiro Hibara, Yutaka Sato, Tsuneaki Watanabe, Kiyomi Abe, Akio Miyao, Misuzu Nosaka-Takahashi, Toshiya Suzuki, Nhung Kim Ta, Sae Shimizu-Sato, Takamasa Suzuki, Atsushi Toyoda, Hirokazu Takahashi, Mikio Nakazono, Yasuo Nagato, Hirohiko Hirochika |
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| Rok vydání: | 2019 |
| Předmět: |
Cell division
Zygote Cellular differentiation Mutant Gene Expression Regulation Enzymologic Transcriptome 03 medical and health sciences 0302 clinical medicine Gene Expression Regulation Plant Arabidopsis Asymmetric cell division Cloning Molecular Protein kinase A Molecular Biology 030304 developmental biology Mitogen-Activated Protein Kinase 6 0303 health sciences biology Asymmetric Cell Division Oryza biology.organism_classification Plants Genetically Modified Cell biology Multicellular organism Seeds 030217 neurology & neurosurgery Cell Division Developmental Biology |
| Zdroj: | SC30202003110007 NARO成果DBa |
| ISSN: | 1477-9129 |
| Popis: | Asymmetric cell division is a key step in cellular differentiation in multicellular organisms. In plants, asymmetric zygotic division produces the apical and basal cells. Mitogen-activated protein kinase (MPK) cascade in Arabidopsis act in asymmetric divisions such as zygotic division and stomatal development, but whether the effect on cellular differentiation by this cascade is direct or indirect following asymmetric division is not clear. Here, we report the analysis of a rice mutant, globular embryo 4 (gle4). In two- and four-cell-stage embryos, asymmetric zygotic division and subsequent cell division patterns were indistinguishable between the wild type and gle4 mutants. However, marker gene expression and transcriptome analyses showed that specification of the basal region was compromised in gle4. We found that GLE4 encodes MPK6 and GLE4/ MPK6 is essential in cellular differentiation rather than in asymmetric zygotic division. Our findings provide a new insight into the role of MPK in plant development. We propose that the regulation of asymmetric zygotic division is separate from the regulation of cellular differentiation that leads to apical-basal polarity. |
| Databáze: | OpenAIRE |
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