| Autor: |
Moshe Szyf, Shafaat A. Rabbani, Haseeb Ahmed Khan, Imrana Tanvir, Rubhana Raqib, Wasif Ali Khan, Sheikh Mohammad Fazle Akbar, Mamun Al-Mahtab, Ze-Guang Han, Michael Hallett, Jian Huang, Ani Arakelian, Matthew Suderman, David Cheishvili, Barbara Stefanska |
| Rok vydání: |
2023 |
| DOI: |
10.1158/1078-0432.22452326 |
| Popis: |
PDF file - 492KB, Supplementary Figure S2. (A) Effect of 5-azaCdR at 1.0 microM concentration on non-transformed NorHep cell growth throughout 20 day-treatment. NorHep cells were seeded at a density of 3 x 105 per a 10-cm tissue culture dish. The medium was replaced with fresh medium containing 1.0 microM 5-azaCdR every 24 h for 3 days and every 48 h for the following 17 days (20 days in total). During the experiment the cells were split every 4-5 days after reaching 80 percent confluence and counted on day 3, 6, 9, 12, 14, and 20. (B-D) Expression and methylation levels of RASAL2 and NENF in NorHep after 1 microM 5-azaCdR treatment for 3 (3d) or 20 days (20d). Relative expression is expressed as a percentage of expression in control non-treated cells. Average methylation state of CpG sites was determined by pyrosequencing in control and treated cells for NENF after 3 days of exposure and for RASAL2 after 20 days (no changes were observed after 3 days as shown in Supplementary Figure S1). All results represent mean � S.D. of two or three independent experiments - ***P < 0.001, **P < 0.01, *P < 0.05. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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