Sensitive detection of DNA methyltransferase activity by transcription-mediated duplex-specific nuclease-assisted cyclic signal amplification
| Autor: | Wen-jing Xu, Chun-yang Zhang, Ya-ping Zeng, Yan Zhang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Site-Specific DNA-Methyltransferase (Adenine-Specific)
Transcription Genetic DNA methyltransferase Catalysis Fluorescence Viral Proteins Transcription (biology) Limit of Detection Materials Chemistry Detection limit Nuclease biology Chemistry Metals and Alloys RNA General Chemistry DNA-Directed RNA Polymerases Molecular biology Surfaces Coatings and Films Electronic Optical and Magnetic Materials Anti-Bacterial Agents Biochemistry Duplex (building) Ceramics and Composites biology.protein Gentamicins Signal amplification |
| Zdroj: | Chemical communications (Cambridge, England). 51(73) |
| ISSN: | 1364-548X |
| Popis: | We develop a sensitive fluorescence method for DNA methyltransferase (MTase) assay based on T7 RNA polymerase-mediated transcription amplification and duplex-specific nuclease (DSN)-assisted cyclic signal amplification. This method exhibits excellent specificity and high sensitivity with a detection limit of 0.015 U mL(-1), and it may be further applied for the screening of antimicrobial drugs. |
| Databáze: | OpenAIRE |
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