Ixodes ticks belonging to the Ixodes ricinus complex encode a family of anticomplement proteins
| Autor: | N. Praet, J. P. Georgin, K. de Fays, Edmond Godfroid, I. Chiappino, Hélène Schroeder, Paul M. Sharp, Alex Bollen, Virginie Daix, Lise Gern, Paul-Pierre Pastoret, Alain Vanderplasschen, Yves Decrem, Gérard Leboulle, Laurent Gillet |
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| Rok vydání: | 2007 |
| Předmět: |
Ixodes ricinus
Molecular Sequence Data Tick Salivary Glands Transcriptome parasitic diseases Genetics Animals Amino Acid Sequence Salivary Proteins and Peptides Molecular Biology Gene Complement Inactivator Proteins Ixodes Sequence Homology Amino Acid biology Ricinus biology.organism_classification Biological Evolution Immunohistochemistry Molecular biology Ixodes scapularis Multigene Family Insect Science Alternative complement pathway Female |
| Zdroj: | Insect Molecular Biology. 16:155-166 |
| ISSN: | 1365-2583 0962-1075 |
| DOI: | 10.1111/j.1365-2583.2006.00710.x |
| Popis: | The alternative pathway of complement is an important innate defence against pathogens including ticks. This component of the immune system has selected for pathogens that have evolved countermeasures. Recently, a salivary protein able to inhibit the alternative pathway was cloned from the American tick Ixodes scapularis (Valenzuela et al., 2000; J. Biol. Chem. 275, 18717–18723). Here, we isolated two different sequences, similar to Isac, from the transcriptome of I. ricinus salivary glands. Expression of these sequences revealed that they both encode secreted proteins able to inhibit the complement alternative pathway. These proteins, called I. ricinus anticomplement (IRAC) protein I and II, are coexpressed constitutively in I. ricinus salivary glands and are upregulated during blood feeding. Also, we demonstrated that they are the products of different genes and not of alleles of the same locus. Finally, phylogenetic analyses demonstrate that ticks belonging to the Ixodes ricinus complex encode a family of relatively small anticomplement molecules undergoing diversification by positive Darwinian selection. |
| Databáze: | OpenAIRE |
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